Optimization of Agrobacterium-Mediated Transformation Parameters for Melastomatacea Spp. Using Green Fluorescent Protein(GFP) as A Reporter

• Main Authors: Thau, Wilson Lym Yong, Abdullah, Janna Ong, Mahmood, Maziah
• Format: Article
• Language: English; English
• Published: Elsevier 2006
• Online Access: http://psasir.upm.edu.my/id/eprint/713/1/Optimization%20of%20Agrobacterium.pdf; http://psasir.upm.edu.my/id/eprint/713/; http://dx.doi.org/10.1016/j.scienta.2006.03.005

Agrobacterium-mediated transformation for both Melastoma malabathricum and Tibouchinu semidecanda were optimized using green fluorescent protein (GFP) as a reporter. The binary vector pCAMBIA1304 harboring the modified green fluorescent protein (mgfp) gene driven by the CuMV 35S promoter was used. P...