Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein

• Main Authors: Tee, Chong Siang, Mahmood, Maziah, Tan, Chon Seng, Abdullah, Mohd Puad
• Format: Article
• Language: English
• Published: Springer 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/22384/1/Selection%20of%20co-transformed%20Dendrobium%20Sonia%2017%20using%20hygromycin%20and%20green%20fluorescent%20protein.pdf; http://psasir.upm.edu.my/id/eprint/22384/; http://link.springer.com/article/10.1007%2Fs10535-011-0128-1

Dendrobium Sonia 17 calluses were used for co-transformation study using particle bombardment. The bombarded transformed callus tissues were selected using half-strength MS medium containing 25 mg dm−3 hygromycin. Expression of green fluorescent protein (GFP) was observed in the callus and protocorm...