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Optimization of biolistic bombardment parameters for Dendrobium sonia 17 calluses using GFP and GUS as the reporter system

Genetic transformation system of Dendrobium Sonia 17 was optimized using green fluorescent protein (GFP) and β-glucuronidase (GUS) gene as the reporter systems. The 35S-sgfp-TYG-nos (p35S) and pSMDFR, carrying sgfp and gusA gene, respectively, were co-bombarded into the calluses. Parameters optimize...

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Bibliographic Details
Main Authors: Tee, Chong Siang, Maziah, Mahmood
Format: Article
Language:English
English
Published: Springer 2005
Online Access:http://psasir.upm.edu.my/id/eprint/18372/1/Optimization%20of%20biolistic%20bombardment%20parameters%20for%20Dendrobium%20sonia%2017%20calluses%20using%20GFP%20and%20GUS%20as%20the%20reporter%20system.pdf
http://psasir.upm.edu.my/id/eprint/18372/
http://dx.doi.org/10.1007/s11240-004-9144-6
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http://psasir.upm.edu.my/id/eprint/18372/1/Optimization%20of%20biolistic%20bombardment%20parameters%20for%20Dendrobium%20sonia%2017%20calluses%20using%20GFP%20and%20GUS%20as%20the%20reporter%20system.pdf
http://psasir.upm.edu.my/id/eprint/18372/
http://dx.doi.org/10.1007/s11240-004-9144-6

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