Strategies in fed-batch cultivation on the production performance of Lactobacillus salivarius I 24 viable cells

• Main Authors: Lim, Chi Ming, Halim, Murni, Abd Rahim, Raha, Ho, Yin Wan, Ariff, Arbakariya
• Format: Article
• Language: English
• Published: Korean Society of Food Science and Technology 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/53897/1/Strategies%20in%20fed-batch%20cultivation%20on%20the%20production%20performance%20of%20Lactobacillus%20salivarius%20I%2024%20viable%20cells.pdf; http://psasir.upm.edu.my/id/eprint/53897/; https://link.springer.com/article/10.1007/s10068-016-0217-1

The potential use of fed-batch cultivation (FBC) for improvement of the production of Lactobacillus salivarius I 24 biomass for subsequent use as probiotics was studied using a 2-L stirredtank bioreactor. Three different constant feeding rates (0.1, 0.05, and 0.033 L/h) were applied in FBCs and their effect on carbon metabolism was evaluated. The carbon flux for cell built-up with reduction in lactic acid synthesis was observed in the fed-batch as compared to the batch cultivation mode. The viable cell number obtained in the constant FBC (CFBC) operated at a feeding rate of 0.05 L/h was 8 times higher (10.7×1010 CFU/mL) than that recorded in the batch cultivation. This gave the viable cell yield based on glucose consumed for CFBC of 26 times higher (11.3×1012 CFU/gGlucose) than the batch cultivation. This study demonstrated CFBC, which is simple with minimal use of process control equipment, has an industrial potential for improvement of probiotic production.