Two-phase fed-batch modification for 48 hour peak expression of hepatitis B surface antigen in Pichia pastoris shake flask system

• Main Authors: Tam, Yew Joon, Allaudin, Zeenathul Nazariah, Morvarid, Akhavan Rezaei, Mohd Lila, Mohd Azmi, Bahaman, Abdul Rani, Lo, Sewn Cen, Tan, Joo Shun
• Format: Article
• Language: English
• Published: Springer 2014
• Online Access: http://psasir.upm.edu.my/id/eprint/35906/1/Two-phase%20fed-batch%20modification%20for%2048%20hour%20peak%20expression%20of%20hepatitis%20B%20surface%20antigen%20in%20Pichia%20pastoris%20shake%20flask%20system.pdf; http://psasir.upm.edu.my/id/eprint/35906/; http://link.springer.com/article/10.2478%2Fs11535-014-0309-y

A study of the Mut+ phenotype for the expression of recombinant hepatitis B surface antigen (HBsAg) in Pichia pastoris strain GS115 using the pPIC3.5K vector with a two-phase fed-batch protocol in a shake flask system is described. Expression levels of HBsAg protein of 6.74 g L−1 Dry Cell Weight (DC...