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Purified T1 lipase, a novel construct of T1 lipase and methods thereoF

The present invention relates to method to substitute second step affinity chromatography with ion exchange chromatography by site-directed mutagenesis at downstream region of GST sequences (H215R and G213R). This is to enable to diferentiate the isoelectric point (pI) value of GST from the pI value...

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Main Authors: Raja Abdul Rahman, Raja Noor Zaliha, Saleh, Abu Bakar, Basri, Mahiran, Leow, Adam Thean Chor, Che Hussi, Che Haznie Ayu
Format: Patent
Published: 2011
Online Access:http://psasir.upm.edu.my/id/eprint/33123/
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spelling my.upm.eprints.331232016-09-27T01:30:52Z http://psasir.upm.edu.my/id/eprint/33123/ Purified T1 lipase, a novel construct of T1 lipase and methods thereoF Raja Abdul Rahman, Raja Noor Zaliha Saleh, Abu Bakar Basri, Mahiran Leow, Adam Thean Chor Che Hussi, Che Haznie Ayu The present invention relates to method to substitute second step affinity chromatography with ion exchange chromatography by site-directed mutagenesis at downstream region of GST sequences (H215R and G213R). This is to enable to diferentiate the isoelectric point (pI) value of GST from the pI value of matured T1 lipase. Also, by obtaining a new construct, new T1 lipase from the above methods, the new construct and T1 lipase was purified using two chromatography steps affinity and ion exchange chromatography. Thus, as mentioned above, the said method provides an advantage to limit or eliminate the second affinity chromatography step. 2011-11-09 Patent NonPeerReviewed Raja Noor Zaliha Raja Abd. Rahman (2011) Purified T1 lipase, a novel construct of T1 lipase and methods thereoF. PI2011700160.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The present invention relates to method to substitute second step affinity chromatography with ion exchange chromatography by site-directed mutagenesis at downstream region of GST sequences (H215R and G213R). This is to enable to diferentiate the isoelectric point (pI) value of GST from the pI value of matured T1 lipase. Also, by obtaining a new construct, new T1 lipase from the above methods, the new construct and T1 lipase was purified using two chromatography steps affinity and ion exchange chromatography. Thus, as mentioned above, the said method provides an advantage to limit or eliminate the second affinity chromatography step.
format Patent
author Raja Abdul Rahman, Raja Noor Zaliha
Saleh, Abu Bakar
Basri, Mahiran
Leow, Adam Thean Chor
Che Hussi, Che Haznie Ayu
spellingShingle Raja Abdul Rahman, Raja Noor Zaliha
Saleh, Abu Bakar
Basri, Mahiran
Leow, Adam Thean Chor
Che Hussi, Che Haznie Ayu
Purified T1 lipase, a novel construct of T1 lipase and methods thereoF
author_facet Raja Abdul Rahman, Raja Noor Zaliha
Saleh, Abu Bakar
Basri, Mahiran
Leow, Adam Thean Chor
Che Hussi, Che Haznie Ayu
author_sort Raja Abdul Rahman, Raja Noor Zaliha
title Purified T1 lipase, a novel construct of T1 lipase and methods thereoF
title_short Purified T1 lipase, a novel construct of T1 lipase and methods thereoF
title_full Purified T1 lipase, a novel construct of T1 lipase and methods thereoF
title_fullStr Purified T1 lipase, a novel construct of T1 lipase and methods thereoF
title_full_unstemmed Purified T1 lipase, a novel construct of T1 lipase and methods thereoF
title_sort purified t1 lipase, a novel construct of t1 lipase and methods thereof
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/33123/
_version_ 1643830786917400576
score 13.154949

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