Optimization of biolistic bombardment parameters for Dendrobium sonia 17 calluses using GFP and GUS as the reporter system
Genetic transformation system of Dendrobium Sonia 17 was optimized using green fluorescent protein (GFP) and β-glucuronidase (GUS) gene as the reporter systems. The 35S-sgfp-TYG-nos (p35S) and pSMDFR, carrying sgfp and gusA gene, respectively, were co-bombarded into the calluses. Parameters optimize...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English English |
| Published: |
Springer
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18372/1/Optimization%20of%20biolistic%20bombardment%20parameters%20for%20Dendrobium%20sonia%2017%20calluses%20using%20GFP%20and%20GUS%20as%20the%20reporter%20system.pdf http://psasir.upm.edu.my/id/eprint/18372/ http://dx.doi.org/10.1007/s11240-004-9144-6 |
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