Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway.
The emergence of gene therapy offers a new paradigm to the field of molecular medicine. However, current viral and non-viral gene transfer vectors are not efficient and often restricted by doselimiting toxicity. Thus, generation of a new gene delivery vector, which is efficient and with good safet...
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Malaysian Medical Association
2010
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Online Access: | http://psasir.upm.edu.my/id/eprint/15952/1/Optimal%20transfection%20conditions%20and%20the%20safety%20profiles%20of%20dextran%20spermine.pdf http://psasir.upm.edu.my/id/eprint/15952/ http://www.mma.org.my/ |
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my.upm.eprints.159522015-11-02T02:07:20Z http://psasir.upm.edu.my/id/eprint/15952/ Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. Rosli, Rozita Wendy-Yeo, W.Y. Veerakumarasivam, Abhinanyu Abdul Rahman, Sabariah Domb, A. J. Abdullah, Syahrilnizam The emergence of gene therapy offers a new paradigm to the field of molecular medicine. However, current viral and non-viral gene transfer vectors are not efficient and often restricted by doselimiting toxicity. Thus, generation of a new gene delivery vector, which is efficient and with good safety profile is highly required. In this study, optimal transfection conditions and safety profile a novel biodegradable cationic polymerdextran-spermine (DSPM) in mouse airways were ascertained. The highest level of gene expression in the lungs of BALB/c mice was detected at D-SPM to plasmid DNA (pDNA) weight ratio (w/w) of 16, with 13.5 µg pDNA. No significant induction of pro-inflammatory cytokines in the broncho alveolar lavage fluids was observed, which implies no overt toxicity occurred in the mouse lungs. In short, these results demonstrate that D-SPM has moderate gene transfer efficiency but with acceptable safety profile in the mouse airways. Malaysian Medical Association 2010-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15952/1/Optimal%20transfection%20conditions%20and%20the%20safety%20profiles%20of%20dextran%20spermine.pdf Rosli, Rozita and Wendy-Yeo, W.Y. and Veerakumarasivam, Abhinanyu and Abdul Rahman, Sabariah and Domb, A. J. and Abdullah, Syahrilnizam (2010) Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. Medical Journal of Malaysia, 65. pp. 116-117. ISSN 0300-5283 http://www.mma.org.my/ English |
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The emergence of gene therapy offers a new paradigm to the field of molecular medicine. However, current viral and non-viral gene transfer vectors are not efficient and often restricted by doselimiting toxicity. Thus, generation of a new gene delivery vector, which is efficient and with good
safety profile is highly required. In this study, optimal
transfection conditions and safety profile a novel biodegradable cationic polymerdextran-spermine (DSPM) in mouse airways were ascertained. The highest level of gene expression in the lungs of BALB/c mice was detected at D-SPM to plasmid DNA (pDNA) weight ratio (w/w) of 16, with 13.5 µg
pDNA. No significant induction of pro-inflammatory cytokines in the broncho alveolar lavage fluids was observed, which implies no overt toxicity occurred in the mouse lungs. In short, these results demonstrate that D-SPM has moderate gene transfer efficiency but with acceptable safety profile in the mouse airways. |
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Rosli, Rozita Wendy-Yeo, W.Y. Veerakumarasivam, Abhinanyu Abdul Rahman, Sabariah Domb, A. J. Abdullah, Syahrilnizam |
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Rosli, Rozita Wendy-Yeo, W.Y. Veerakumarasivam, Abhinanyu Abdul Rahman, Sabariah Domb, A. J. Abdullah, Syahrilnizam Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. |
author_facet |
Rosli, Rozita Wendy-Yeo, W.Y. Veerakumarasivam, Abhinanyu Abdul Rahman, Sabariah Domb, A. J. Abdullah, Syahrilnizam |
author_sort |
Rosli, Rozita |
title |
Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. |
title_short |
Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. |
title_full |
Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. |
title_fullStr |
Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. |
title_full_unstemmed |
Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. |
title_sort |
optimal transfection conditions and the safety profiles of dextran spermine/plasmid dna as potential gene transfer vector to mouse airway. |
publisher |
Malaysian Medical Association |
publishDate |
2010 |
url |
http://psasir.upm.edu.my/id/eprint/15952/1/Optimal%20transfection%20conditions%20and%20the%20safety%20profiles%20of%20dextran%20spermine.pdf http://psasir.upm.edu.my/id/eprint/15952/ http://www.mma.org.my/ |
_version_ |
1643826076723445760 |
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13.160551 |