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Meat species identification and Halal authentication analysis using mitochondrial DNA.

A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the mitochondrial genes was developed for beef (Bos taurus), pork (Sus scrofa), buffalo (Bubalus bubali), quail (Coturnix coturnix), chicken (Gallus gallus), goat (Capra hircus), rabbit (Oryctolagus cuniculus) species identifi...

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Main Authors: Murugaiah, Chandrika, Mohd Noor, Zainon, Mastakim, Maimunah, Bilung, Lesley Maurice, Selamat, Jinap, Radu, Son
Format: Article
Language:English
English
Published: Elsevier 2009
Online Access:http://psasir.upm.edu.my/id/eprint/15580/1/Meat%20species%20identification%20and%20Halal%20authentication%20analysis%20using%20mitochondrial%20DNA.pdf
http://psasir.upm.edu.my/id/eprint/15580/
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spelling my.upm.eprints.155802015-10-22T23:35:11Z http://psasir.upm.edu.my/id/eprint/15580/ Meat species identification and Halal authentication analysis using mitochondrial DNA. Murugaiah, Chandrika Mohd Noor, Zainon Mastakim, Maimunah Bilung, Lesley Maurice Selamat, Jinap Radu, Son A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the mitochondrial genes was developed for beef (Bos taurus), pork (Sus scrofa), buffalo (Bubalus bubali), quail (Coturnix coturnix), chicken (Gallus gallus), goat (Capra hircus), rabbit (Oryctolagus cuniculus) species identification and Halal authentication. PCR products of 359-bp were successfully obtained from the cyt b gene of these six meats. AluI, BsaJI, RsaI, MseI, and BstUI enzymes were identified as potential restriction endonucleases to differentiate the meats. The genetic differences within the cyt b gene among the meat were successfully confirmed by PCR-RFLP. A reliable typing scheme of species which revealed the genetic differences among the species was developed. Elsevier 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15580/1/Meat%20species%20identification%20and%20Halal%20authentication%20analysis%20using%20mitochondrial%20DNA.pdf Murugaiah, Chandrika and Mohd Noor, Zainon and Mastakim, Maimunah and Bilung, Lesley Maurice and Selamat, Jinap and Radu, Son (2009) Meat species identification and Halal authentication analysis using mitochondrial DNA. Meat Science, 83 (1). pp. 57-61. ISSN 0309-1740 10.1016/j.meatsci.2009.03.015 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the mitochondrial genes was developed for beef (Bos taurus), pork (Sus scrofa), buffalo (Bubalus bubali), quail (Coturnix coturnix), chicken (Gallus gallus), goat (Capra hircus), rabbit (Oryctolagus cuniculus) species identification and Halal authentication. PCR products of 359-bp were successfully obtained from the cyt b gene of these six meats. AluI, BsaJI, RsaI, MseI, and BstUI enzymes were identified as potential restriction endonucleases to differentiate the meats. The genetic differences within the cyt b gene among the meat were successfully confirmed by PCR-RFLP. A reliable typing scheme of species which revealed the genetic differences among the species was developed.
format Article
author Murugaiah, Chandrika
Mohd Noor, Zainon
Mastakim, Maimunah
Bilung, Lesley Maurice
Selamat, Jinap
Radu, Son
spellingShingle Murugaiah, Chandrika
Mohd Noor, Zainon
Mastakim, Maimunah
Bilung, Lesley Maurice
Selamat, Jinap
Radu, Son
Meat species identification and Halal authentication analysis using mitochondrial DNA.
author_facet Murugaiah, Chandrika
Mohd Noor, Zainon
Mastakim, Maimunah
Bilung, Lesley Maurice
Selamat, Jinap
Radu, Son
author_sort Murugaiah, Chandrika
title Meat species identification and Halal authentication analysis using mitochondrial DNA.
title_short Meat species identification and Halal authentication analysis using mitochondrial DNA.
title_full Meat species identification and Halal authentication analysis using mitochondrial DNA.
title_fullStr Meat species identification and Halal authentication analysis using mitochondrial DNA.
title_full_unstemmed Meat species identification and Halal authentication analysis using mitochondrial DNA.
title_sort meat species identification and halal authentication analysis using mitochondrial dna.
publisher Elsevier
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/15580/1/Meat%20species%20identification%20and%20Halal%20authentication%20analysis%20using%20mitochondrial%20DNA.pdf
http://psasir.upm.edu.my/id/eprint/15580/
_version_ 1643825969324097536
score 13.159267

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