Amidination of lipase with hydrophobic imidoesters

• Main Authors: Basri, M., Ampon, K., Yunus, W. M. Z., Razak, C. N. A., Salleh, A. B.
• Format: Article
• Published: Wiley 1992
• Online Access: http://psasir.upm.edu.my/id/eprint/113042/; https://aocs.onlinelibrary.wiley.com/doi/10.1007/BF02636112

Lipase from Candida rugosa was chemically modified by amidination with imidoester hydrochlorides of different hydrophobicity. The modified enzyme showed a higher ester synthesis activity but a lower ester hydrolysis activity compared with the native enzyme. The maximum specific activity of the modif...