Development of a catalytically stable and efficient lipase through an increase in hydrophobicity of the oxyanion residue

• Main Authors: Abdul Wahab, Roswanira, Basri, Mahiran, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Abdul Rahman, Mohd. Basyaruddin, Leow, Thean Chor
• Format: Article
• Published: Elsevier B.V. 2015
• Subjects: QD Chemistry
• Online Access: http://eprints.utm.my/id/eprint/58281/; http://dx.doi.org/10.1016/j.molcatb.2015.10.003

In-silico and empirical site-directed substitutions of oxyanion Q114 of the wildtype T1 lipase with that of hydrophobic Leu and Met residues were carried out to afford the Q114L and Q114M lipases, respectively. Using the esterification production of menthyl butyrate as a reaction model, evaluation o...