Synthesis of fatty esters by polyethylene glycol-modified lipase

• Main Authors: Basri, Mahiran, Ampon, Kamaruzaman, Wan Yunus, Wan Md. Zin, Abdul Razak, Che Nyonya, Salleh, Abu Bakar
• Format: Article
• Language: English
• Published: Society of Chemical Industry 1995
• Online Access: http://psasir.upm.edu.my/id/eprint/22572/1/Synthesis%20of%20fatty%20esters%20by%20polyethylene%20glycol-modified%20lipase.pdf; http://psasir.upm.edu.my/id/eprint/22572/; http://onlinelibrary.wiley.com/doi/10.1002/jctb.280640103/abstract

Monomethoxypolyethylene glycols (PEG) of molecular masses 1900 and 5000 were activated using p-nitrophenyl chloroformate to form PEG–nitrophenyl carbonates (activated PEG) with high yield (96–98%). The activated PEG was covalently attached to Candida rugosa lipase. Increasing the molar ratio of acti...