Shifting the pH profiles of Staphylococcus epidermidis lipase (SEL) and Staphylococcus hyicus lipase (SHL) through generating chimeric lipases by DNA shuffling strategy

• Main Authors: Wan Hasan, Wan Atiqah Najiah, Nezhad, Nima Ghahremani, Yaacob, Mohd Adilin, Salleh, Abu Bakar, Raja Abdul Rahman, Raja Noor Zaliha, Leow, Thean Chor
• Format: Article
• Published: Springer Science and Business Media 2024
• Online Access: http://psasir.upm.edu.my/id/eprint/112036/; https://link.springer.com/article/10.1007/s11274-024-03927-x

Enzymes are often required to function in a particular reaction condition by the industrial procedure. In order to identify critical residues affecting the optimum pH of Staphylococcal lipases, chimeric lipases from homologous lipases were generated via a DNA shuffling strategy. Chimeric 1 included...