Directed evolution of recombinant c-terminal truncated staphylococcus epidermidis lipase AT2 for the enhancement of thermostability

• Main Authors: Veno, Jiivittha, Ahmad Kamarudin, Nor Hafizah, Mohamad Ali, Mohd Shukuri, Masomian, Malihe, Raja Abd. Rahman, Raja Noor Zaliha
• Format: Article
• Language: English
• Published: Multidisciplinary Digital Publishing Institute 2017
• Online Access: http://psasir.upm.edu.my/id/eprint/61489/1/Directed%20evolution%20of%20recombinant%20c-terminal%20truncated%20staphylococcus%20epidermidis%20lipase%20AT2%20for%20the%20enhancement%20of%20thermostability.pdf; http://psasir.upm.edu.my/id/eprint/61489/; https://www.mdpi.com/1422-0067/18/11/2202

In the industrial processes, lipases are expected to operate at temperatures above 45 °C and could retain activity in organic solvents. Hence, a C-terminal truncated lipase from Staphylococcus epidermis AT2 (rT-M386) was engineered by directed evolution. A mutant with glycine-to-cysteine substitutio...