Environment-Friendly Approach to Isolate Ascorbic Acid from Plants and Micro-identified Using Fourier Transform Infrared Spectroscopy
Vitamin C [ascorbic acid (AA)] is an imperative antioxidant that humans require. Fruits and vegetables commonly contain AA. The application of titrimetric or chromatographic methods is generally performed to analyze AA; nevertheless, these approaches have several disadvantages such as being very co...
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Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
American Chemical Society
2024
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Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/44376/3/Environment.pdf http://ir.unimas.my/id/eprint/44376/ https://pubs.acs.org/doi/10.1021/acsfoodscitech.3c00575 https://doi.org/10.1021/acsfoodscitech.3c00575 |
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Summary: | Vitamin C [ascorbic acid (AA)] is an imperative antioxidant that humans require. Fruits and vegetables commonly
contain AA. The application of titrimetric or chromatographic methods is generally performed to analyze AA; nevertheless, these approaches have several disadvantages such as being very costly for chromatographic methods and exhibiting food color interference
upon using titration techniques. This study cruises the application of microwave treatment on AA compounds of plant samples and identified the AA level using Fourier transform infrared (FTIR) spectroscopy. The purposes of this study were to establish a validated method for the microwave-assisted isolation (MAI) of AA from plant samples. Validation studies such as linearity,
selectivity, sensitivity, detection, and quantitation limits were evaluated using a standard AA solution. These methods were also
applied for AA quantitation in oranges, guava, and spinach. The methods were selective and sensitive when applied to the standard
solution. The FTIR spectroscopy approach was more sensitive, with lower values for detection limit (13.76 nmol·L−1
) and
quantitation limit (41.69 nmol·L−1
), and more accurate with an error of less than 1%. The imperative AA groups are also identified,
such as the hydroxyl groups found at 3297 nm−1
. In contrast, the aromatic group of AA can be obtained at 2084 cm−1
, although the
absorption intensity is weak. The 1635 cm−1 atom is related to the carbonyl group (−C�O). The validated method was applied to
investigate the AA level in plant samples, and all of them showed identical peaks with the standard, showing that the MAI has
isolated the AA completely. |
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