Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
The Japanese encephalitis virus (JEV), a leading cause of encephalitis, exists as quasispecies in clinical isolates. Using a limiting dilution method combined with immunohistochemistry to detect viral antigens, 10 biological clones were isolated and purified from a clinical JEV isolate (CNS138/9) de...
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my.unimas.ir.334982021-03-30T08:02:16Z http://ir.unimas.my/id/eprint/33498/ Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model Shu, Pin Yu Kien, Chai Ong Soon, Hao Tan David, Perera Kum, Thong Wong R Medicine (General) The Japanese encephalitis virus (JEV), a leading cause of encephalitis, exists as quasispecies in clinical isolates. Using a limiting dilution method combined with immunohistochemistry to detect viral antigens, 10 biological clones were isolated and purified from a clinical JEV isolate (CNS138/9) derived from an autopsy brain. These biological clones were tested for neurovirulence in SK-N-MC and NIE-115 neuronal cells, and a 2-week-old, footpad-infected, JE mouse model. Nine clones were found to be neurovirulent; one clone neuroattenuated. Although further studies are needed to determine genotypic differences, if any, in these clones, the limiting dilution purification and neurovirulence testing methods described herein should be useful for phenotypic studies of quasispecies of neurotropic viruses in general, and JEV and other flaviviruses in particular. 2020 Article PeerReviewed text en http://ir.unimas.my/id/eprint/33498/1/Shu%20Pin%20Yu.pdf Shu, Pin Yu and Kien, Chai Ong and Soon, Hao Tan and David, Perera and Kum, Thong Wong (2020) Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model. Neurology Asia, 25 (3). pp. 279-284. ISSN 1823-6138 https://www.neurology-asia.org/articles/neuroasia-2020-25(3)-279.pdf |
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R Medicine (General) Shu, Pin Yu Kien, Chai Ong Soon, Hao Tan David, Perera Kum, Thong Wong Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model |
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The Japanese encephalitis virus (JEV), a leading cause of encephalitis, exists as quasispecies in clinical isolates. Using a limiting dilution method combined with immunohistochemistry to detect viral antigens, 10 biological clones were isolated and purified from a clinical JEV isolate (CNS138/9) derived from an autopsy brain. These biological clones were tested for neurovirulence in SK-N-MC and NIE-115 neuronal cells, and a 2-week-old, footpad-infected, JE mouse model. Nine clones were found to be neurovirulent; one clone neuroattenuated. Although further studies are needed to determine genotypic differences, if any, in these clones, the limiting dilution purification and neurovirulence testing methods described herein should be useful for phenotypic studies of quasispecies of neurotropic viruses in general, and JEV and other flaviviruses in particular. |
format |
Article |
author |
Shu, Pin Yu Kien, Chai Ong Soon, Hao Tan David, Perera Kum, Thong Wong |
author_facet |
Shu, Pin Yu Kien, Chai Ong Soon, Hao Tan David, Perera Kum, Thong Wong |
author_sort |
Shu, Pin Yu |
title |
Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model |
title_short |
Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model |
title_full |
Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model |
title_fullStr |
Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model |
title_full_unstemmed |
Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model |
title_sort |
japanese encephalitis virus: biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model |
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2020 |
url |
http://ir.unimas.my/id/eprint/33498/1/Shu%20Pin%20Yu.pdf http://ir.unimas.my/id/eprint/33498/ https://www.neurology-asia.org/articles/neuroasia-2020-25(3)-279.pdf |
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