Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model

• Main Authors: Shu, Pin Yu, Kien, Chai Ong, Soon, Hao Tan, David, Perera, Kum, Thong Wong
• Format: Article
• Language: English
• Published: 2020
• Subjects: R Medicine (General)
• Online Access: http://ir.unimas.my/id/eprint/33498/1/Shu%20Pin%20Yu.pdf; http://ir.unimas.my/id/eprint/33498/; https://www.neurology-asia.org/articles/neuroasia-2020-25(3)-279.pdf

The Japanese encephalitis virus (JEV), a leading cause of encephalitis, exists as quasispecies in clinical isolates. Using a limiting dilution method combined with immunohistochemistry to detect viral antigens, 10 biological clones were isolated and purified from a clinical JEV isolate (CNS138/9) de...