Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods

The discoveries on new resources of natural antioxidant from plants have attracted increasing interest due to high potential of antioxidant capacity. In this paper, antioxidant activity of Cosmos caudatus extracts was investigated by using different types of extraction methods which were maceration...

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Main Author: Nur Afeefa, Mohd Khairi
Format: Undergraduates Project Papers
Language:English
Published: 2013
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Online Access:http://umpir.ump.edu.my/id/eprint/7027/1/19.ANTIOXIDANT%20ACTIVITY%20OF%20Cosmos%20caudatus%20EXTRACTS.pdf
http://umpir.ump.edu.my/id/eprint/7027/
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spelling my.ump.umpir.70272023-04-11T01:11:20Z http://umpir.ump.edu.my/id/eprint/7027/ Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods Nur Afeefa, Mohd Khairi TX Home economics The discoveries on new resources of natural antioxidant from plants have attracted increasing interest due to high potential of antioxidant capacity. In this paper, antioxidant activity of Cosmos caudatus extracts was investigated by using different types of extraction methods which were maceration extraction, Soxhlet extraction and ultrasonic-assisted extraction using 70% acetone (v/v) as solvent. The antioxidant activity and ascorbic acid content from each method was determined by 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) assay and High Performance Liquid Chromatography (HPLC) respectively. The results show that the three extraction methods exhibit significant value of ascorbic acid content. Ultrasonic-assisted extraction was the efficient method as it exhibits the highest amount of ascorbic acid content with 26.590 mg ascorbic acid equivalent per 1 g sample (mg AAE/g) with the corresponding value of 42.208% of its antioxidant activity after 60 minutes extraction. This is followed by Soxhlet extraction (13.527 mg AAE/g) and maceration extraction (5.190 mg AAE/g) within 8 hour and 24 hour extraction time respectively. Analysis by HPLC also shows that C. caudatus extract using ultrasonicassisted extraction has the highest vitamin C content (2.440 mg AAE/g) followed by Soxhlet extraction (0.968 mg AAE/g) and maceration extraction (0.739 mg AAE/g). For further study, optimization of the ultrasonic extraction method is proposed to achieve the optimum condition in extracting bioactive compounds from C. caudatus. 2013-02 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/7027/1/19.ANTIOXIDANT%20ACTIVITY%20OF%20Cosmos%20caudatus%20EXTRACTS.pdf Nur Afeefa, Mohd Khairi (2013) Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TX Home economics
spellingShingle TX Home economics
Nur Afeefa, Mohd Khairi
Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods
description The discoveries on new resources of natural antioxidant from plants have attracted increasing interest due to high potential of antioxidant capacity. In this paper, antioxidant activity of Cosmos caudatus extracts was investigated by using different types of extraction methods which were maceration extraction, Soxhlet extraction and ultrasonic-assisted extraction using 70% acetone (v/v) as solvent. The antioxidant activity and ascorbic acid content from each method was determined by 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) assay and High Performance Liquid Chromatography (HPLC) respectively. The results show that the three extraction methods exhibit significant value of ascorbic acid content. Ultrasonic-assisted extraction was the efficient method as it exhibits the highest amount of ascorbic acid content with 26.590 mg ascorbic acid equivalent per 1 g sample (mg AAE/g) with the corresponding value of 42.208% of its antioxidant activity after 60 minutes extraction. This is followed by Soxhlet extraction (13.527 mg AAE/g) and maceration extraction (5.190 mg AAE/g) within 8 hour and 24 hour extraction time respectively. Analysis by HPLC also shows that C. caudatus extract using ultrasonicassisted extraction has the highest vitamin C content (2.440 mg AAE/g) followed by Soxhlet extraction (0.968 mg AAE/g) and maceration extraction (0.739 mg AAE/g). For further study, optimization of the ultrasonic extraction method is proposed to achieve the optimum condition in extracting bioactive compounds from C. caudatus.
format Undergraduates Project Papers
author Nur Afeefa, Mohd Khairi
author_facet Nur Afeefa, Mohd Khairi
author_sort Nur Afeefa, Mohd Khairi
title Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods
title_short Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods
title_full Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods
title_fullStr Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods
title_full_unstemmed Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods
title_sort antioxidant activity of cosmos caudatus extracts by using different types of extraction methods
publishDate 2013
url http://umpir.ump.edu.my/id/eprint/7027/1/19.ANTIOXIDANT%20ACTIVITY%20OF%20Cosmos%20caudatus%20EXTRACTS.pdf
http://umpir.ump.edu.my/id/eprint/7027/
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score 13.144533