A bioanalytical method for quantification of telmisartan in rat plasma; development, validation and application to pharmacokinetic study
The telmisartan was determined in a rat plasma using developed and validated a reversed-phase high performance liquid chromatographic (HPLC). The pre-treatment of the plasma sample involving liquid-liquid extraction using ethanol as the extracting solvent. The HPLC method validation has been shown a...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
A and V publication
2021
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/25861/1/A%20Bioanalytical%20Method%20for%20Quantification%20of%20Telmisartan%20in%20rat%20Plasma%20Development%20Validation%20and%20Application%20to%20Pharmacokinetic%20Study.pdf http://umpir.ump.edu.my/id/eprint/25861/ https: //doi.org/10.52711/0974-360X.2021.00379 |
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| Summary: | The telmisartan was determined in a rat plasma using developed and validated a reversed-phase high performance liquid chromatographic (HPLC). The pre-treatment of the plasma sample involving liquid-liquid extraction using ethanol as the extracting solvent. The HPLC method validation has been shown a linear calibration curve over a plasma concentrations range of 0.7 to 10µg/mL with a correlation coefficient of 0.9979, the limit of detection and the limit of quantification were determined to be 0.025µg/ml and 0.07µg/ml, respectively. The precision and accuracy were in an acceptable limit. The pharmacokinetic parameters of telmisartan were adequately evaluated following a single oral dose (4mg/kg) in Sprague-Dawley rats. The results observed conclude that the developed bioanalytical HPLC method is appropriate and applicable as an analytical tool in the pharmacokinetic study of telmisartan. |
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