Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles

• Main Authors: Sharifah Fathiyah, Sy Mohamad, Farhan, Mohd Said, Mimi Sakinah, A. M., Shahril, Mohamad
• Format: Article
• Language: English
• Published: Department of Chemistry, Universitas Gadjah Mada Yogyakarta 2019
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/25736/1/Effect%20of%20key%20parameters%20on%20jacalin%20extraction.pdf; http://umpir.ump.edu.my/id/eprint/25736/; https://doi.org/10.22146/ijc.39705; https://doi.org/10.22146/ijc.39705

This study demonstrates the extraction of jacalin from crude extract of jackfruit seeds using anionic surfactant based reverse micellar system, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. Effects of various parameters, such as, NaCl concentration (0.05 – 1 M), pH of aqueous phase (pH 4 – 10), AOT concentration (5 – 150 mM), contact time (5 – 30 min) and phase volume ratio (0.5-5) on the transfer efficiency of jacalin was evaluated by changing one factor at a time (OFAT) while keeping the other parameters constant. A maximum of 83% of protein transfer was achieved after equal volume of organic and aqueous phase was stirred for 20 min using 20 mM AOT at pH 5 aqueous phase containing 0.1 M NaCl. The findings demonstrated AOT reverse micellar system as a promising and effective method to extract and purify jacalin from crude protein mixture.