Relationship between free-radical scavenging activities and total phenolic compound of two selected timber species in Malaysia

• Main Author: Nur Amalina Aziz Khan
• Format: Undergraduate Final Project Report
• Published: 2014
• Online Access: http://discol.umk.edu.my/id/eprint/5919/

Two plant species were selected, which are Hevea Brasiliensis (Rubberwood) and Shorea spp. (Meranti) for determination of total phenolic compound and the free-radical scavenging activities. In this study, the total phenolic compound was determined by using Folin-ciocalteau reagent. Free-radical scavenging activitity was determine by using DPPH (1,1- dipheny1-2-picryl hydrazyl) radical. Free radical scavenging activities was determined by using DPPH radical which is expressed as EC50 (µg/m1). Total phenolic content varied between the species. Hevea Brasiliensis (Rubberwood) have higher total phenolic content compared to Shorea spp. (Meranti), which is 316.444 mg GAE/mg and 117.555 mg GAE/mg respectively. Result showed that the Hevea Brasiliensis (Rubberwood) has lower EC50 value compare to Shorea spp. (Meranti). So, Hevea Brasiliensis (Rubberwood) has high free radical scavenging activity. The relationship between free-radical scavenging activities and total phenolic compound was interpreted by using graph. There are correlationship between free-radical scavenging activities and total phenolic compound. Higher total phenolic compound show high free radical scavenging activities.