Protein precipitation method for salivary proteins and rehydration buffer for two-dimensional electrophoresis

• Main Authors: Jessie, K., Hashim, Onn Haji, Rahim, Z.H.A.
• Format: Article
• Language: English
• Published: 2008
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/3437/1/Protein_Precipitation_Method_for_Salivary_Proteins_and_Rehydration_Buffer_for_Two-Dimensional_Electrophoresis.pdf; http://eprints.um.edu.my/3437/

Precipitants for salivary proteins and rehydration buffers for two-dimensional electrophoresis (2-DE) analysis were, respectively compared and evaluated. Five different protein precipitants: TCA, TCA-acetone-DTT, TCA-acetone-mercaptoethanol, acetone and alcohol were used to precipitate proteins of t...