Optimal protein extraction methods from diverse sample types for protein profiling by using Two-Dimensional Electrophoresis (2DE)

• Main Authors: Tan, A.A., Azman, S.N., Abdul Rani, N.R., Kua, B.C., Sasidharan, S., Kiew, L.V., Othman, N., Noordin, R., Chen, Y.
• Format: Article
• Language: English
• Published: 2011
• Subjects: Q Science (General); QR Microbiology; RB Pathology; RZ Other systems of medicine
• Online Access: http://eprints.um.edu.my/2643/1/Kiew_LV_2011.docx; http://eprints.um.edu.my/2643/

There is a great diversity of protein samples types and origins, therefore the optimal procedure for each sample type must be determined empirically. In order to obtain a reproducible and complete sample presentation which view as many proteins as possible on the desired 2DE gel, it is critical to...