Function of PD-L1 in antitumor immunity of glioma cells
Human glioma is a highly fatal tumor with a significant feature of immune suppression. The functions of PD-L1 refer to co-simulation and immune regulation. To investigate expression and functional activity of PD-L1 in human glioma cell in vivo and in vitro. Expressions of PD-L1mRNA and protein in th...
Saved in:
Main Authors: | , , , , |
---|---|
Format: | Article |
Published: |
Elsevier
2017
|
Subjects: | |
Online Access: | http://eprints.um.edu.my/19107/ http://dx.doi.org/10.1016/j.sjbs.2015.06.025 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.um.eprints.19107 |
---|---|
record_format |
eprints |
spelling |
my.um.eprints.191072018-09-05T02:59:36Z http://eprints.um.edu.my/19107/ Function of PD-L1 in antitumor immunity of glioma cells Lou, Y.L. Shi, J. Guo, D. Qureshi, A.K. Song, L.J. Q Science (General) QD Chemistry R Medicine Human glioma is a highly fatal tumor with a significant feature of immune suppression. The functions of PD-L1 refer to co-simulation and immune regulation. To investigate expression and functional activity of PD-L1 in human glioma cell in vivo and in vitro. Expressions of PD-L1mRNA and protein in the human glioma cell line were analyzed with quantitative RT-PCR and flow cytometer; and then expression of PD-L1 in tissue specimens of 10 glioma patients was treated with immunohistochemical analysis; glioma cell and allogeneic CD4+ and CD8+ T cells were co-cultured, and cytokine IFN-γ, IL-2 and IL-10 in cultured supernatant fluid were determined with ELISA; upon blocking the interaction between glioma cell and the immune cell with PD-L1 monoclonal antibody (5H1), surface markers on immune cells were analyzed using flow cytometer. All human glioma cell lines constitutively expressed PD-L1, and IFN-γ induced glioma cell to highly express PD-L1. It was shown through immunohistochemical analysis that glioma specimen expressed PD-L1, while expression of PD-L1 was not observed in normal tissue and normal human brain near the tumor location. The release of IFN-γ and IL-2 was inhibited, while IL-10 was increased slightly. Glioma cell may escape from immune recognition and injury with the help of PD-L1, which is a significant pathogenic mechanism of glioma. Elsevier 2017 Article PeerReviewed Lou, Y.L. and Shi, J. and Guo, D. and Qureshi, A.K. and Song, L.J. (2017) Function of PD-L1 in antitumor immunity of glioma cells. Saudi Journal of Biological Sciences, 24 (4). pp. 803-807. ISSN 1319-562X http://dx.doi.org/10.1016/j.sjbs.2015.06.025 doi:10.1016/j.sjbs.2015.06.025 |
institution |
Universiti Malaya |
building |
UM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Malaya |
content_source |
UM Research Repository |
url_provider |
http://eprints.um.edu.my/ |
topic |
Q Science (General) QD Chemistry R Medicine |
spellingShingle |
Q Science (General) QD Chemistry R Medicine Lou, Y.L. Shi, J. Guo, D. Qureshi, A.K. Song, L.J. Function of PD-L1 in antitumor immunity of glioma cells |
description |
Human glioma is a highly fatal tumor with a significant feature of immune suppression. The functions of PD-L1 refer to co-simulation and immune regulation. To investigate expression and functional activity of PD-L1 in human glioma cell in vivo and in vitro. Expressions of PD-L1mRNA and protein in the human glioma cell line were analyzed with quantitative RT-PCR and flow cytometer; and then expression of PD-L1 in tissue specimens of 10 glioma patients was treated with immunohistochemical analysis; glioma cell and allogeneic CD4+ and CD8+ T cells were co-cultured, and cytokine IFN-γ, IL-2 and IL-10 in cultured supernatant fluid were determined with ELISA; upon blocking the interaction between glioma cell and the immune cell with PD-L1 monoclonal antibody (5H1), surface markers on immune cells were analyzed using flow cytometer. All human glioma cell lines constitutively expressed PD-L1, and IFN-γ induced glioma cell to highly express PD-L1. It was shown through immunohistochemical analysis that glioma specimen expressed PD-L1, while expression of PD-L1 was not observed in normal tissue and normal human brain near the tumor location. The release of IFN-γ and IL-2 was inhibited, while IL-10 was increased slightly. Glioma cell may escape from immune recognition and injury with the help of PD-L1, which is a significant pathogenic mechanism of glioma. |
format |
Article |
author |
Lou, Y.L. Shi, J. Guo, D. Qureshi, A.K. Song, L.J. |
author_facet |
Lou, Y.L. Shi, J. Guo, D. Qureshi, A.K. Song, L.J. |
author_sort |
Lou, Y.L. |
title |
Function of PD-L1 in antitumor immunity of glioma cells |
title_short |
Function of PD-L1 in antitumor immunity of glioma cells |
title_full |
Function of PD-L1 in antitumor immunity of glioma cells |
title_fullStr |
Function of PD-L1 in antitumor immunity of glioma cells |
title_full_unstemmed |
Function of PD-L1 in antitumor immunity of glioma cells |
title_sort |
function of pd-l1 in antitumor immunity of glioma cells |
publisher |
Elsevier |
publishDate |
2017 |
url |
http://eprints.um.edu.my/19107/ http://dx.doi.org/10.1016/j.sjbs.2015.06.025 |
_version_ |
1643690889606856704 |
score |
13.188404 |