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Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis

Sample preparation for scanning electron microscope analysis involves reagents and equipment that are expensive and often hazardous. Here we demonstrate a circumvention of Osmium tetroxide and critical point drying, greatly reducing the duration, complexity and cost of the process. We captured early...

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Main Authors: Osahor, A., Deekonda, K., Lee, C.W., Sim, E.U.H., Radu, A., Narayanan, K.
Format: Article
Published: Elsevier 2017
Subjects:
QH301 Biology
Online Access:http://eprints.um.edu.my/17581/
https://doi.org/10.1016/j.ab.2017.07.008
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spelling my.um.eprints.175812017-07-31T06:28:35Z http://eprints.um.edu.my/17581/ Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis Osahor, A. Deekonda, K. Lee, C.W. Sim, E.U.H. Radu, A. Narayanan, K. QH301 Biology Sample preparation for scanning electron microscope analysis involves reagents and equipment that are expensive and often hazardous. Here we demonstrate a circumvention of Osmium tetroxide and critical point drying, greatly reducing the duration, complexity and cost of the process. We captured early stage interactions of invasive-bacteria and HeLa cells during the process of bacteria-mediated gene delivery and illustrate sufficient clarity can be obtained using this procedure to preserve and clearly visualize relevant cellular structures. This protocol is significantly cheaper and easier to adapt compared to conventional methods, and will allow routine preparation/viewing of eukaryotic or bacterial samples for basic morphological studies. Elsevier 2017 Article PeerReviewed Osahor, A. and Deekonda, K. and Lee, C.W. and Sim, E.U.H. and Radu, A. and Narayanan, K. (2017) Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis. Analytical Biochemistry, 534. pp. 46-48. ISSN 0003-2697 https://doi.org/10.1016/j.ab.2017.07.008 DOI: 10.1016/j.ab.2017.07.008
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QH301 Biology
spellingShingle QH301 Biology
Osahor, A.
Deekonda, K.
Lee, C.W.
Sim, E.U.H.
Radu, A.
Narayanan, K.
Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis
description Sample preparation for scanning electron microscope analysis involves reagents and equipment that are expensive and often hazardous. Here we demonstrate a circumvention of Osmium tetroxide and critical point drying, greatly reducing the duration, complexity and cost of the process. We captured early stage interactions of invasive-bacteria and HeLa cells during the process of bacteria-mediated gene delivery and illustrate sufficient clarity can be obtained using this procedure to preserve and clearly visualize relevant cellular structures. This protocol is significantly cheaper and easier to adapt compared to conventional methods, and will allow routine preparation/viewing of eukaryotic or bacterial samples for basic morphological studies.
format Article
author Osahor, A.
Deekonda, K.
Lee, C.W.
Sim, E.U.H.
Radu, A.
Narayanan, K.
author_facet Osahor, A.
Deekonda, K.
Lee, C.W.
Sim, E.U.H.
Radu, A.
Narayanan, K.
author_sort Osahor, A.
title Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis
title_short Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis
title_full Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis
title_fullStr Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis
title_full_unstemmed Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis
title_sort rapid preparation of adherent mammalian cells for basic scanning electron microscopy (sem) analysis
publisher Elsevier
publishDate 2017
url http://eprints.um.edu.my/17581/
https://doi.org/10.1016/j.ab.2017.07.008
_version_ 1643690458142998528
score 13.159267

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