Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis

• Main Authors: Andrew, Osahor, Karthik, Deekonda, Choon, Weng Lee, Ui, Edmund Hang Sim, Aurelian, Radu, Kumaran, Narayanan
• Format: E-Article
• Published: Academic Press Inc. 2017
• Subjects: QR Microbiology
• Online Access: http://ir.unimas.my/id/eprint/17178/; http://www.sciencedirect.com/science/article/pii/S0003269717302920; https://doi.org/10.1016/j.ab.2017.07.008

Sample preparation for scanning electron microscope analysis involves reagents and equipment that are expensive and often hazardous. Here we demonstrate a circumvention of Osmium tetroxide and critical point drying, greatly reducing the duration, complexity and cost of the process. We captured early...