Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis

• Main Authors: Osahor, A., Deekonda, K., Lee, C.W., Sim, E.U.H., Radu, A., Narayanan, K.
• Format: Article
• Published: Elsevier 2017
• Subjects: QH301 Biology
• Online Access: http://eprints.um.edu.my/17581/; https://doi.org/10.1016/j.ab.2017.07.008

Sample preparation for scanning electron microscope analysis involves reagents and equipment that are expensive and often hazardous. Here we demonstrate a circumvention of Osmium tetroxide and critical point drying, greatly reducing the duration, complexity and cost of the process. We captured early...