A detection of escherichia coli and salmonella sp. In raw coconut milk using MPN-PCR method / Noor Syafiqah Jalaluddin

• Main Author: Jalaluddin, Noor Syafiqah
• Format: Student Project
• Language: English
• Published: Faculty of Applied Sciences 2015
• Subjects: QR Microbiology; Bacteria
• Online Access: http://ir.uitm.edu.my/id/eprint/23906/1/PPb_NOOR%20SYAFIQAH%20JALALUDDIN%20AS%20N%2015_5.pdf; http://ir.uitm.edu.my/id/eprint/23906/

Little is known on the biohazard level of microorganisms in raw products. The main purpose of this study was to investigate the prevalence and quantification of Escherichia coli and Salmonella sp. in the raw coconut milk samples by using Most Probable Number-Polymerase Chain Reaction (MPN-PCR) method. A total of ten samples of raw coconut milks were purchased randomly from several wet market and hypermarket in Kuala Pilah and Senawang areas. A total of 10% (1/10) samples from both markets showed positive result of Escherichia coli while 100% (10/10) samples showed positive result of Salmonella sp. The estimated quantities of positive turbid tubes from the wet market were ranging from 2.0x105 MPN/g to 2.4x107 MPN/g. The estimated quantities of positive turbid tubes from hypermarket were ranging from 2.8x105 MPN/g to 2.4x107 MPN/g. In this study, the presumptive amount of Escherichia coli in wet market samples was ranging from 3.0x104 MPN/g to 9.4x104 MPN/g while Salmonella sp. was ranging from 3.0x104 MPN/g to 4.4x105 MPN/g. Based on the result, it showed that Escherichia coli did not occur in hypermarket samples. Meanwhile, Salmonella sp. was ranging from 3.0x104 MPN/g to 2.4x107 MPN/g. The outcome of this study showed that the presence of microbial contaminants that indicates poor milk quality, which requires immediate consideration as it can pose serious health risk to consumers.