DNA methylation of disrupted in schizophrenia 1 (disc1) gene in schizophrenia using methylight taqman assay

• Main Authors: Abd. Rahim, Nour El Huda, Rahim, Mohd Nabil Fikri, Ku Zaifah, Norsidah, Mohd Noor, Hanisah, Abdullah, Kartini, Talib, Norlelawati A.
• Format: Conference or Workshop Item
• Language: English; English
• Published: Kulliyyah of Medicine 2016
• Subjects: R Medicine (General); RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
• Online Access: http://irep.iium.edu.my/52015/19/52015.pdf; http://irep.iium.edu.my/52015/20/52015_DNA%20methylation_Complete.pdf; http://irep.iium.edu.my/52015/

Introduction: Significant evidences from functional studies have shown that DISC1 gene has a role in the pathogenesis of schizophrenia, although the basis of the genetic defect has yet to be established. There has been a shift of emphasis from DISC1 gene variations to other genetic defect such as copy number and epigenetic, both of which have not been well investigated. Thus, the aim of this study is to examine the DNA methylation status of DISC1 gene in patients with schizophrenia. Methods: In this study, 239 subjects were included, 117 schizophrenia patients and 122 healthy controls. Genomic DNA was derived from peripheral blood and bisulfite converted. The DNA methylation level was quantitatively measured by Methylight Taqman analysis. Sociodemographic and the clinical parameters were noted. The severity of the clinical symptoms was assessed using Positive and Negative Syndrome Scale (PANSS). Results: The mean age and gender distribution of the study groups were similar. There were no significant differences in the methylation level of DISC1 between the patients and control groups When patients were compared by age, duration of illness, age at diagnosis, body mass index, smoking status, PANSS score, and types of antipsychotic treatment, the DNA methylation level of DISC1, did not show any significant difference (p>0.05). Conclusions: This study found no significant difference in methylation level of DISC1 gene between schizophrenia patients and healthy control. Therefore it is suggested that aberrant DNA methylation of DISC1 most probably does not contribute to the pathogenesis of schizophrenia.