The single nucleotide polymorphisms rs11761556 and rs12706832 of the leptin gene are associated with type 2 diabetes mellitus in the Iraqi population
This study was conducted to assess the potential association between leptin (LEP) gene polymorphisms and type 2 diabetes mellitus (T2DM) in Iraqi patients. Genomic DNA was extracted from 120 diabetic subjects and 100 controls. Three specific PCR fragments were designed to flank three highly frequent...
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Institut za Bioloska Istrazivanja
2021
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Online Access: | http://eprints.utm.my/id/eprint/94531/1/MusaferKarar2021_TheSingleNucleotidePolymorphisms.pdf http://eprints.utm.my/id/eprint/94531/ http://dx.doi.org/10.2298/ABS210129005M |
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my.utm.945312022-03-31T14:55:27Z http://eprints.utm.my/id/eprint/94531/ The single nucleotide polymorphisms rs11761556 and rs12706832 of the leptin gene are associated with type 2 diabetes mellitus in the Iraqi population Musafer, K. N. J. Huyop, F. Z. Ewadh, M. J. Supriyanto, E. Al-Thuwaini, T. M. Al-Shuhaib, M. B. S. Q Science (General) This study was conducted to assess the potential association between leptin (LEP) gene polymorphisms and type 2 diabetes mellitus (T2DM) in Iraqi patients. Genomic DNA was extracted from 120 diabetic subjects and 100 controls. Three specific PCR fragments were designed to flank three highly frequent single nucleotide polymorphism (SNP)s within LEP, rs11761556, rs12706832 and rs2167270. The amplified loci were genotyped by PCR-single-strand conformation polymorphism (SSCP) followed by Sanger sequencing for representative genotypes. Logistic regression analysis was performed to detect the association between the targeted genetic variants and T2DM. PCR-SSCP genotyping showed three banding patterns for all three targeted SNPs. Individuals with the AA genotype in both rs11761556 and rs12706832 SNPs showed significantly higher (P<0.05) body mass index (BMI), waist circumference (WC), fasting blood glucose (FBG), hemoglobin A1c (HbA1c), homeostatic model assessment for insulin resistance (HOMA-IR), insulin, low-density lipoprotein cholesterol (LDL-C) and triglyceride (TG) values than those with other genotypes. Association analysis revealed that individuals with the A allele exhibited a greater risk of T2DM. Data of the present investigation indicated that both rs11761556 and rs12706832 SNPs exerted a noticeable association with T2DM. The study suggests implementing both rs11761556 and rs12706832 SNPs in the early detection of T2DM. Institut za Bioloska Istrazivanja 2021 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/94531/1/MusaferKarar2021_TheSingleNucleotidePolymorphisms.pdf Musafer, K. N. J. and Huyop, F. Z. and Ewadh, M. J. and Supriyanto, E. and Al-Thuwaini, T. M. and Al-Shuhaib, M. B. S. (2021) The single nucleotide polymorphisms rs11761556 and rs12706832 of the leptin gene are associated with type 2 diabetes mellitus in the Iraqi population. Archives of Biological Sciences, 73 (1). ISSN 0354-4664 http://dx.doi.org/10.2298/ABS210129005M DOI: 10.2298/ABS210129005M |
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Q Science (General) Musafer, K. N. J. Huyop, F. Z. Ewadh, M. J. Supriyanto, E. Al-Thuwaini, T. M. Al-Shuhaib, M. B. S. The single nucleotide polymorphisms rs11761556 and rs12706832 of the leptin gene are associated with type 2 diabetes mellitus in the Iraqi population |
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This study was conducted to assess the potential association between leptin (LEP) gene polymorphisms and type 2 diabetes mellitus (T2DM) in Iraqi patients. Genomic DNA was extracted from 120 diabetic subjects and 100 controls. Three specific PCR fragments were designed to flank three highly frequent single nucleotide polymorphism (SNP)s within LEP, rs11761556, rs12706832 and rs2167270. The amplified loci were genotyped by PCR-single-strand conformation polymorphism (SSCP) followed by Sanger sequencing for representative genotypes. Logistic regression analysis was performed to detect the association between the targeted genetic variants and T2DM. PCR-SSCP genotyping showed three banding patterns for all three targeted SNPs. Individuals with the AA genotype in both rs11761556 and rs12706832 SNPs showed significantly higher (P<0.05) body mass index (BMI), waist circumference (WC), fasting blood glucose (FBG), hemoglobin A1c (HbA1c), homeostatic model assessment for insulin resistance (HOMA-IR), insulin, low-density lipoprotein cholesterol (LDL-C) and triglyceride (TG) values than those with other genotypes. Association analysis revealed that individuals with the A allele exhibited a greater risk of T2DM. Data of the present investigation indicated that both rs11761556 and rs12706832 SNPs exerted a noticeable association with T2DM. The study suggests implementing both rs11761556 and rs12706832 SNPs in the early detection of T2DM. |
format |
Article |
author |
Musafer, K. N. J. Huyop, F. Z. Ewadh, M. J. Supriyanto, E. Al-Thuwaini, T. M. Al-Shuhaib, M. B. S. |
author_facet |
Musafer, K. N. J. Huyop, F. Z. Ewadh, M. J. Supriyanto, E. Al-Thuwaini, T. M. Al-Shuhaib, M. B. S. |
author_sort |
Musafer, K. N. J. |
title |
The single nucleotide polymorphisms rs11761556 and rs12706832 of the leptin gene are associated with type 2 diabetes mellitus in the Iraqi population |
title_short |
The single nucleotide polymorphisms rs11761556 and rs12706832 of the leptin gene are associated with type 2 diabetes mellitus in the Iraqi population |
title_full |
The single nucleotide polymorphisms rs11761556 and rs12706832 of the leptin gene are associated with type 2 diabetes mellitus in the Iraqi population |
title_fullStr |
The single nucleotide polymorphisms rs11761556 and rs12706832 of the leptin gene are associated with type 2 diabetes mellitus in the Iraqi population |
title_full_unstemmed |
The single nucleotide polymorphisms rs11761556 and rs12706832 of the leptin gene are associated with type 2 diabetes mellitus in the Iraqi population |
title_sort |
single nucleotide polymorphisms rs11761556 and rs12706832 of the leptin gene are associated with type 2 diabetes mellitus in the iraqi population |
publisher |
Institut za Bioloska Istrazivanja |
publishDate |
2021 |
url |
http://eprints.utm.my/id/eprint/94531/1/MusaferKarar2021_TheSingleNucleotidePolymorphisms.pdf http://eprints.utm.my/id/eprint/94531/ http://dx.doi.org/10.2298/ABS210129005M |
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13.18916 |