The mechanistic role of active site residues in non-stereo haloacid dehalogenase E (DehE)

• Main Authors: Zainal Abidin, Muhammad Hasanuddin, Abd. Halim, Khairul Bariyyah, Huyop, Fahrul, Tengku Abdul Hamid, Tengku Haziyamin, Abdul Wahab, Roswanira, Abdul Hamid, Azzmer Azzar
• Format: Article
• Published: Elsevier Inc. 2019
• Subjects: QD Chemistry
• Online Access: http://eprints.utm.my/id/eprint/87453/; http://dx.doi.org/10.1016/j.jmgm.2019.05.003

Dehalogenase E (DehE) is a non-stereospecific enzyme produced by the soil bacterium, Rhizobium sp. RC1. Till now, the catalytic mechanism of DehE remains unclear although several literature concerning its structure and function are available. Since DehE is non-stereospecific, the enzyme was hypothes...