An S188V Mutation Alters Substrate Specificity of Non- Stereospecific alpha-Haloalkanoic Acid Dehalogenase E (DehE)

• Main Authors: Abdul Hamid, Azzmer Azzar, Tengku Abdul Hamid, Tengku Haziyamin, Abdul Wahab, Roswanira, Omar, Mohd. Shahir Shamsir, Huyop, Fahrul
• Format: Article
• Language: English
• Published: Penerbit UTM Press 2015
• Subjects: QD Chemistry
• Online Access: http://eprints.utm.my/id/eprint/57794/1/RoswaniraAbdulWahab2015_AnS188VMutationAltersSubstrateSpecificity.pdf; http://eprints.utm.my/id/eprint/57794/; http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121687

The non-stereospecific α-haloalkanoic acid dehalogenase E (DehE) degrades many halogenated compounds but is ineffective against β-halogenated compounds such as 3-chloropropionic acid (3CP). Using molecular dynamics (MD) simulations and site-directed mutagenesis we show here that introducing the muta...