Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method

• Main Authors: Kek, Siok Peng, Chin, Nyuk Ling, Tan, Sheau Wei, Yusof, Yus Aniza, Chua, Lee Suan
• Format: Article
• Published: Blackwell Publishing Ltd 2018
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/86688/; http://dx.doi.org/10.1111/ijfs.13840

Four different DNA protocols were compared to obtain the best method for DNA extraction of bees in honey samples. The efficiency was evaluated in terms of DNA concentration and purity, PCR amplification capacity targeting mitochondrial 16S rRNA gene, and execution time. The most efficient DNA extrac...