Synthesis, functionalization, characterization, and: in vitro evaluation of robust pH-sensitive CFNs-PA-CaCO3
The preparation, characterization, and application of Papain (PA) conjugated CaCO3-coated cobalt ferrite nanoparticles (CFNs-PA-CaCO3) is reported. Papain was covalently attached onto silanized cobalt ferrite nanoparticles (CFNs-APS) and then coated with robust pH sensitive CaCO3. It was characteriz...
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Royal Society of Chemistry
2016
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my.utm.746002017-11-29T23:58:41Z http://eprints.utm.my/id/eprint/74600/ Synthesis, functionalization, characterization, and: in vitro evaluation of robust pH-sensitive CFNs-PA-CaCO3 Nasiri, Mahtab Tabrizi, Sayed Ali Hassanzadeh Almaki, Javad Hamzehalipour Nasiri, Rozita Idris, Ani Dabagh, Shadab TP Chemical technology The preparation, characterization, and application of Papain (PA) conjugated CaCO3-coated cobalt ferrite nanoparticles (CFNs-PA-CaCO3) is reported. Papain was covalently attached onto silanized cobalt ferrite nanoparticles (CFNs-APS) and then coated with robust pH sensitive CaCO3. It was characterized by FTIR, XRD, TEM, FESEM, and TGA. The release behaviour of PA from functionalized nanoparticles in acidic and physiological pH was investigated. However, the rate of PA release was slow and enhanced with time at physiological pH; the results illustrate that acidic conditions disassembles the coating, which caused a rapid release of PA. The biocompatibility evaluation of CFNs/CFNs-CaCO3 and the cytotoxicity of free PA/CFNs-PA was carried out in vitro on human breast cancer cells (MDA-MB-231) and human skin fibroblast cells (HSF 1184) by performing an MTT assay. An improved cytotoxic effect was obtained for PA conjugated cobalt ferrites in comparison with free PA. The synthesized delivery system was perfectly tolerable to the blood and cells in concentrations below 200 μg mL-1. Investigations were followed out to measure the time and dose dependent cellular uptake of functionalized nanoparticles via the Prussian blue staining method under an inverted microscope, which indicated that functionalized nanoparticles showed internalization properties. The results conveyed that the synthesized system is a promising biomaterial for future biomedical applications, mainly cancer therapy. The present in vitro findings require comparison with those of in vivo studies. Royal Society of Chemistry 2016 Article PeerReviewed Nasiri, Mahtab and Tabrizi, Sayed Ali Hassanzadeh and Almaki, Javad Hamzehalipour and Nasiri, Rozita and Idris, Ani and Dabagh, Shadab (2016) Synthesis, functionalization, characterization, and: in vitro evaluation of robust pH-sensitive CFNs-PA-CaCO3. RSC Advances, 6 (87). pp. 84217-84230. ISSN 2046-2069 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84986596463&doi=10.1039%2fc6ra14918a&partnerID=40&md5=795ed885f14006b7d966d1c883480ef9 |
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TP Chemical technology Nasiri, Mahtab Tabrizi, Sayed Ali Hassanzadeh Almaki, Javad Hamzehalipour Nasiri, Rozita Idris, Ani Dabagh, Shadab Synthesis, functionalization, characterization, and: in vitro evaluation of robust pH-sensitive CFNs-PA-CaCO3 |
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The preparation, characterization, and application of Papain (PA) conjugated CaCO3-coated cobalt ferrite nanoparticles (CFNs-PA-CaCO3) is reported. Papain was covalently attached onto silanized cobalt ferrite nanoparticles (CFNs-APS) and then coated with robust pH sensitive CaCO3. It was characterized by FTIR, XRD, TEM, FESEM, and TGA. The release behaviour of PA from functionalized nanoparticles in acidic and physiological pH was investigated. However, the rate of PA release was slow and enhanced with time at physiological pH; the results illustrate that acidic conditions disassembles the coating, which caused a rapid release of PA. The biocompatibility evaluation of CFNs/CFNs-CaCO3 and the cytotoxicity of free PA/CFNs-PA was carried out in vitro on human breast cancer cells (MDA-MB-231) and human skin fibroblast cells (HSF 1184) by performing an MTT assay. An improved cytotoxic effect was obtained for PA conjugated cobalt ferrites in comparison with free PA. The synthesized delivery system was perfectly tolerable to the blood and cells in concentrations below 200 μg mL-1. Investigations were followed out to measure the time and dose dependent cellular uptake of functionalized nanoparticles via the Prussian blue staining method under an inverted microscope, which indicated that functionalized nanoparticles showed internalization properties. The results conveyed that the synthesized system is a promising biomaterial for future biomedical applications, mainly cancer therapy. The present in vitro findings require comparison with those of in vivo studies. |
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Article |
author |
Nasiri, Mahtab Tabrizi, Sayed Ali Hassanzadeh Almaki, Javad Hamzehalipour Nasiri, Rozita Idris, Ani Dabagh, Shadab |
author_facet |
Nasiri, Mahtab Tabrizi, Sayed Ali Hassanzadeh Almaki, Javad Hamzehalipour Nasiri, Rozita Idris, Ani Dabagh, Shadab |
author_sort |
Nasiri, Mahtab |
title |
Synthesis, functionalization, characterization, and: in vitro evaluation of robust pH-sensitive CFNs-PA-CaCO3 |
title_short |
Synthesis, functionalization, characterization, and: in vitro evaluation of robust pH-sensitive CFNs-PA-CaCO3 |
title_full |
Synthesis, functionalization, characterization, and: in vitro evaluation of robust pH-sensitive CFNs-PA-CaCO3 |
title_fullStr |
Synthesis, functionalization, characterization, and: in vitro evaluation of robust pH-sensitive CFNs-PA-CaCO3 |
title_full_unstemmed |
Synthesis, functionalization, characterization, and: in vitro evaluation of robust pH-sensitive CFNs-PA-CaCO3 |
title_sort |
synthesis, functionalization, characterization, and: in vitro evaluation of robust ph-sensitive cfns-pa-caco3 |
publisher |
Royal Society of Chemistry |
publishDate |
2016 |
url |
http://eprints.utm.my/id/eprint/74600/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-84986596463&doi=10.1039%2fc6ra14918a&partnerID=40&md5=795ed885f14006b7d966d1c883480ef9 |
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1643656889188220928 |
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13.188404 |