Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes

• Main Authors: Sathishkumar, Palanivel, Mythili, Arumugam, Hadibarata, Tony, Jayakumar, Rajarajeswaran, Kanthimathi, M. S., Thayumanavan, Palvannan, Ponraj, Mohanadoss, Salim, Mohd. Razman, Mohd. Yusoff, Abdull Rahim
• Format: Article
• Language: English
• Published: The Royal Society of Chemistry 2014
• Subjects: TA Engineering (General). Civil engineering (General)
• Online Access: http://eprints.utm.my/id/eprint/53295/1/PalanivelSathishkumar2014_Laccasemediateddiclofenactransformation.pdf; http://eprints.utm.my/id/eprint/53295/; http://dx.doi.org/10.1039/c3ra46014b

Diclofenac is recently considered as one of the most devastating environmental pollutants, because of its biomagnification in the food chain which leads to potential harmful effects on non-targeted organisms. This study describes the optimized laccase mediated diclofenac transformation using response surface methodology (RSM) and cytotoxicity testing on mouse fibroblast 3T3-L1 preadipocytes. Three factors (laccase, syringaldehyde, reaction time) were used to optimize the diclofenac transformation. The optimum level of laccase, syringaldehyde and reaction time was found to be 1.91 U mL -1, 187 μg and 51 min for diclofenac transformation (20 mg L -1). The cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes showed that a maximum of 67.9% cell death occurred at 72 h treatment with diclofenac (200 μg mL-1), while the cells treated with laccase treated diclofenac (LTD) showed less toxicity on the cells. These findings can be addressed for the removal of diclofenac toxicity