Fabrication of BSA loaded poly (Caprolactone) (PCL)/ hydroxyapatite (HA) composite microsphere for tissue engineering application
In this study, PCL microsphere has been synthesised using the double emulsion technique and a freeze dry technique. Bovine Serum Albumin (BSA) was added as a model protein and Hydroxyapatite (HA) to enhance osteoconductivity properties of the microsphere. Poly (caprolactone) (PCL)-based microspheres...
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| Main Authors: | , , |
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| Format: | Article |
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Trans Tech Publications, Switzerland
2014
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/52846/ https://dx.doi.org/10.4028/www.scientific.net/AMR.1030-1032.82 |
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| Summary: | In this study, PCL microsphere has been synthesised using the double emulsion technique and a freeze dry technique. Bovine Serum Albumin (BSA) was added as a model protein and Hydroxyapatite (HA) to enhance osteoconductivity properties of the microsphere. Poly (caprolactone) (PCL)-based microspheres were synthesized and characterized. Microspheres were produced with 5%, 10%, and 15% (w/v) PCL solution (30mL) by dissolving different amounts of PCL in dichloromethane (DCM). Microspheres produced from 5% (w/v) PCL concentration had an average diameter of 100 – 120 μm. Bovine serum albumin (BSA) (0.04% w/v) and 10% w/w of HA powder were added into the microspheres produced from 5% (w/v) PCL concentration. The microsphere was then characterized using different techniques and in vitro release study of BSA was conducted. Prepared microsphere showed good potential for bone tissue engineering. |
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