Enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semidefined medium
Xanthan gum, a heteropolysaccharide produced by Xanthomonas campasteris, is a widely used biopolymer in food industries. The production process is highly influenced by the type and concentration of the different carbon and nitrogen source as well as other medium components. The aim of this work was...
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my.utm.290502019-03-05T01:34:11Z http://eprints.utm.my/id/eprint/29050/ Enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semidefined medium El Enshasy, Hesham Then, Charles Othman, Nor Zalina Al Homosany, H. Sabry, M. Sarmidi, Mohamad Roji Aziz, Ramlan QD Chemistry Xanthan gum, a heteropolysaccharide produced by Xanthomonas campasteris, is a widely used biopolymer in food industries. The production process is highly influenced by the type and concentration of the different carbon and nitrogen source as well as other medium components. The aim of this work was to develop an economic industrial medium for industrial production. In shake flask level, the maximal production of xanthan of 19.9 gL -1 was achieved by excluding ammonium nitrate from the industrial medium and making new medium formulation composed of (sucrose, soybean meal, ammonium phosphate and magnesium sulphate). Further optimization in the production process was achieved by transferring the process to 16-L bioreactor and cultivation under controlled pH condition. The maximal volumetric and specific xanthan production [Y P/X] obtained were 28 gL -1 and 11.06 g g -1, respectively. Thus, the semi-defined medium formulation developed in this work could be better and alternatively used for large scale production process for xanthan production when compared to other published media in respect to yield and cost. Academic Journals 2011-02-07 Article PeerReviewed El Enshasy, Hesham and Then, Charles and Othman, Nor Zalina and Al Homosany, H. and Sabry, M. and Sarmidi, Mohamad Roji and Aziz, Ramlan (2011) Enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semidefined medium. African Journal of Biotechnology, 10 (6). pp. 1029-1038. ISSN 1684-5315 https://www.ajol.info/index.php/ajb/article/view/92716 |
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QD Chemistry El Enshasy, Hesham Then, Charles Othman, Nor Zalina Al Homosany, H. Sabry, M. Sarmidi, Mohamad Roji Aziz, Ramlan Enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semidefined medium |
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Xanthan gum, a heteropolysaccharide produced by Xanthomonas campasteris, is a widely used biopolymer in food industries. The production process is highly influenced by the type and concentration of the different carbon and nitrogen source as well as other medium components. The aim of this work was to develop an economic industrial medium for industrial production. In shake flask level, the maximal production of xanthan of 19.9 gL -1 was achieved by excluding ammonium nitrate from the industrial medium and making new medium formulation composed of (sucrose, soybean meal, ammonium phosphate and magnesium sulphate). Further optimization in the production process was achieved by transferring the process to 16-L bioreactor and cultivation under controlled pH condition. The maximal volumetric and specific xanthan production [Y P/X] obtained were 28 gL -1 and 11.06 g g -1, respectively. Thus, the semi-defined medium formulation developed in this work could be better and alternatively used for large scale production process for xanthan production when compared to other published media in respect to yield and cost. |
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El Enshasy, Hesham Then, Charles Othman, Nor Zalina Al Homosany, H. Sabry, M. Sarmidi, Mohamad Roji Aziz, Ramlan |
author_facet |
El Enshasy, Hesham Then, Charles Othman, Nor Zalina Al Homosany, H. Sabry, M. Sarmidi, Mohamad Roji Aziz, Ramlan |
author_sort |
El Enshasy, Hesham |
title |
Enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semidefined medium |
title_short |
Enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semidefined medium |
title_full |
Enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semidefined medium |
title_fullStr |
Enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semidefined medium |
title_full_unstemmed |
Enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semidefined medium |
title_sort |
enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semidefined medium |
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Academic Journals |
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2011 |
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http://eprints.utm.my/id/eprint/29050/ https://www.ajol.info/index.php/ajb/article/view/92716 |
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