Cloning and expression of pullulanase gene from locally isolated bacillus SP

• Main Authors: Nik Mahmood, Nik Azmi, Md. Illias, Rosli, Md. Salleh, Madihah, Hassan, Osman, Kamaruddin, Kamarulzaman, Shahab, Neelam
• Format: Monograph
• Language: English
• Published: Universiti Teknologi Malaysia 2006
• Subjects: Q Science (General)
• Online Access: http://eprints.utm.my/id/eprint/2754/1/74189.pdf; http://eprints.utm.my/id/eprint/2754/; https://www.researchgate.net/publication/254655826_Cloning_and_Expression_of_Pullulanase_a_Gene_from_Locally_Isolated_Bacillus

Bacterial pullulanase represents one of th e starch-degrading enzymes that are widely used in the starch processing indu stry along with amylases. Amylases hydrolyze a -(1,4 )-glycosidic linkage in starch to produce a mixture of glucose , maltooligo sacchari de and limited a-dextrin. All the remaining a -(1,6)-glycosid ic branches in the products are hydrolyzed by p ullulanase. This is an advantage t o improve glucose production by coupling pullulanase and amylase in the p rocess. As such, many pullulana e enzyme has been isolated and one has been showing optimum pH of 10-10.5 which is suitable for use in dishwasher detergent additive in removal of star ch stain. We have recently iso lated a few bacterias that have shown potentially pullulanase producers by the holo-zone in pullulan-plate assay. One of them, we named Bacillus –1 sho ws a bigger holo-zone among others, Bacillus- 1 is highly active in pH more than 7. The enzyme also shows a mo derate activity to wards starch that may be indicates be side hydrolyzes a -(1,6)-glycosidic linkage in starch, it also hydrolyzes a -(1,4)- glycosidi c simi lar to a -amylase. Unfortunately the enzyme from wild-type bacteria is in lower yield an d in this studies, we intend to clone and sequence the pullulanase gene and also expressed the gene in a high expression system to be able to produce in a high yield before characterizing expressed protein.