Cloning and expression of 3-hydroxybutyrate dehydrogenase gene from locally isolated Pseudomonas sp.

• Main Authors: Nik Mahmod, Nik Azmi, Mohd Illias, Rosli, Syed Muhammad, Syed Annuar Fauad, H. D., Habib Abdullah Fikri, S., Wan Nor Azlinda, Lau, S. Y.
• Format: Article
• Language: English
• Published: Universiti Malaysia Sabah 2003
• Subjects: T Technology (General)
• Online Access: http://eprints.utm.my/id/eprint/6922/1/NikAzmiNikMahmod2003_CloningAndExpressionOf3-hydroxybutyrateDehydrogenase.pdf; http://eprints.utm.my/id/eprint/6922/

The 3-HBDH gene from Pseudomonas sp. was amplified, cloned, and sequenced. The deduced amino acid sequence was highly matched with 3-HBDH sequences in DDBJ/GenBank/EMBL. But Pseudomonas sp. 3-HBDH was lack of the C-terminal region found in mammalian enzymes containing a lipid-binding domain that is...