Effects of filtration volume using powdered activated charcoal on the recovery of L (+)-lactic acid from sago starch

• Main Authors: Bujang, Kopli, Ang, Shin Ying, Awang Adeni, Dayang Salwani
• Format: Conference or Workshop Item
• Language: English
• Published: 2006
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/215/1/KopliBBujang2006_Effectsoffiltrationvolumeusing.pdf; http://eprints.utm.my/id/eprint/215/

In this preliminary study, fine powdered activated charcoal (PAC) was used to recover lactic acid from the impurities existed in fermentation broth with different volume size of crude lactic acid. 200 g of activated charcoal in 1N hydrochloric acid (HCl) was packed into glass columns (49mm in diameter), followed by deionized water until pH around 6. Next, 0.5 L (or 2.5 mL/g PAC) and 1.0 L (or 5.0 mL/g PAC) of autoclaved cell-free fermentation broth was added to the glass columns, followed by 0.5 L and 1.0 L of deionized water respectively. It is observed that the efficiency of UV light absorbing compounds removal at ?200 and ?260 (%) is 94.42, 97.61 and 92.55, 97.56 respectively for 0.5L and 1.0L of fermentation broth. Color removal efficiency is observed to be the same (more than 99%) whereas lactic acid recovery increases from 13.56% to 46.43% with the ascend of sample (fermentation broth) volume loaded. On the other hand, the recovery of lactic acid by deionized water (0.5 and 1.0L) is 84.04% and 51.86% respectively. It gives a total recovery of 97.60 and 98.29% respectively for both parameters. Protein and glucose recove