Expression and characterization of trichoderma virens UKM-1 endochitinase in escherichia coli

A gene encoding endochitinase from Trichoderma virens UKM-1 was cloned and expressed in E. coli BL21 (DE3). Both the endochitinase gene and its cDNA sequences were obtained. The endochitinase gene encodes 430 amino acids from an open reading frame comprising of 1,690 bp nucleotide sequence with thre...

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Main Authors: Alias, Nadiawati, Mahadi, Nor Muhammad, Abdul Murad, Abdul Munir, Abu Bakar, Farah Diba, Nik Mahmood, Nik Azmi, Md. Illias, Rosli
Format: Article
Published: Kluwer Academic Publishers 2009
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Online Access:http://eprints.utm.my/id/eprint/14308/
http://dx.doi.org/10.1007/s11274-008-9924-y
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spelling my.utm.143082011-08-26T05:03:03Z http://eprints.utm.my/id/eprint/14308/ Expression and characterization of trichoderma virens UKM-1 endochitinase in escherichia coli Alias, Nadiawati Mahadi, Nor Muhammad Abdul Murad, Abdul Munir Abu Bakar, Farah Diba Nik Mahmood, Nik Azmi Md. Illias, Rosli QH426 Genetics A gene encoding endochitinase from Trichoderma virens UKM-1 was cloned and expressed in E. coli BL21 (DE3). Both the endochitinase gene and its cDNA sequences were obtained. The endochitinase gene encodes 430 amino acids from an open reading frame comprising of 1,690 bp nucleotide sequence with three introns. The endochitinase was expressed as soluble and active enzyme at 20°C when induced with 1 mM IPTG. Maximum activity was observed at 4 h of post-induction time. SDS-PAGE showed that the purified endochitinase exhibited a single band with molecular weight of 42 kDa. Biochemical characterization of the enzyme displayed a near neutral pH characteristic with an optimum pH at 6.0 and optimum temperature at 50°C. The enzyme is stable between pH 3.0-7.0 and is able to retain its activity from 30 to 60°C. The presence of Mg 2+ and Ca2+ ions increased the enzyme activity up to 20%. The purified enzyme has a strong affinity towards colloidal chitin and low effect on ethyl cellulose and D-cellubiose which are non-chitin related substrates. HPLC analysis from the chitin hydrolysis showed the release of (GlcNAc)3, (GlcNAc)2 and GlcNAc, in which (GlcNAc) 2 was the main product. Kluwer Academic Publishers 2009 Article PeerReviewed Alias, Nadiawati and Mahadi, Nor Muhammad and Abdul Murad, Abdul Munir and Abu Bakar, Farah Diba and Nik Mahmood, Nik Azmi and Md. Illias, Rosli (2009) Expression and characterization of trichoderma virens UKM-1 endochitinase in escherichia coli. World Journal of Microbiology and Biotechnology, 25 (4). pp. 561-572. ISSN 0959-3993 http://dx.doi.org/10.1007/s11274-008-9924-y doi:10.1007/s11274-008-9924-y
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QH426 Genetics
spellingShingle QH426 Genetics
Alias, Nadiawati
Mahadi, Nor Muhammad
Abdul Murad, Abdul Munir
Abu Bakar, Farah Diba
Nik Mahmood, Nik Azmi
Md. Illias, Rosli
Expression and characterization of trichoderma virens UKM-1 endochitinase in escherichia coli
description A gene encoding endochitinase from Trichoderma virens UKM-1 was cloned and expressed in E. coli BL21 (DE3). Both the endochitinase gene and its cDNA sequences were obtained. The endochitinase gene encodes 430 amino acids from an open reading frame comprising of 1,690 bp nucleotide sequence with three introns. The endochitinase was expressed as soluble and active enzyme at 20°C when induced with 1 mM IPTG. Maximum activity was observed at 4 h of post-induction time. SDS-PAGE showed that the purified endochitinase exhibited a single band with molecular weight of 42 kDa. Biochemical characterization of the enzyme displayed a near neutral pH characteristic with an optimum pH at 6.0 and optimum temperature at 50°C. The enzyme is stable between pH 3.0-7.0 and is able to retain its activity from 30 to 60°C. The presence of Mg 2+ and Ca2+ ions increased the enzyme activity up to 20%. The purified enzyme has a strong affinity towards colloidal chitin and low effect on ethyl cellulose and D-cellubiose which are non-chitin related substrates. HPLC analysis from the chitin hydrolysis showed the release of (GlcNAc)3, (GlcNAc)2 and GlcNAc, in which (GlcNAc) 2 was the main product.
format Article
author Alias, Nadiawati
Mahadi, Nor Muhammad
Abdul Murad, Abdul Munir
Abu Bakar, Farah Diba
Nik Mahmood, Nik Azmi
Md. Illias, Rosli
author_facet Alias, Nadiawati
Mahadi, Nor Muhammad
Abdul Murad, Abdul Munir
Abu Bakar, Farah Diba
Nik Mahmood, Nik Azmi
Md. Illias, Rosli
author_sort Alias, Nadiawati
title Expression and characterization of trichoderma virens UKM-1 endochitinase in escherichia coli
title_short Expression and characterization of trichoderma virens UKM-1 endochitinase in escherichia coli
title_full Expression and characterization of trichoderma virens UKM-1 endochitinase in escherichia coli
title_fullStr Expression and characterization of trichoderma virens UKM-1 endochitinase in escherichia coli
title_full_unstemmed Expression and characterization of trichoderma virens UKM-1 endochitinase in escherichia coli
title_sort expression and characterization of trichoderma virens ukm-1 endochitinase in escherichia coli
publisher Kluwer Academic Publishers
publishDate 2009
url http://eprints.utm.my/id/eprint/14308/
http://dx.doi.org/10.1007/s11274-008-9924-y
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score 13.18916