Expression and characterization of trichoderma virens UKM-1 endochitinase in escherichia coli

• Main Authors: Alias, Nadiawati, Mahadi, Nor Muhammad, Abdul Murad, Abdul Munir, Abu Bakar, Farah Diba, Nik Mahmood, Nik Azmi, Md. Illias, Rosli
• Format: Article
• Published: Kluwer Academic Publishers 2009
• Subjects: QH426 Genetics
• Online Access: http://eprints.utm.my/id/eprint/14308/; http://dx.doi.org/10.1007/s11274-008-9924-y

A gene encoding endochitinase from Trichoderma virens UKM-1 was cloned and expressed in E. coli BL21 (DE3). Both the endochitinase gene and its cDNA sequences were obtained. The endochitinase gene encodes 430 amino acids from an open reading frame comprising of 1,690 bp nucleotide sequence with thre...