In vitro surface efficacy of CaP-based anodised titanium for bone implants
This study examined the effect of changes to the surface of CaP-based anodised titanium on osteogenesis and antimicrobial activity in vitro. The present work is proposed to investigate the mechanical stability of two different CaP/TiO2 micromorphology-based coatings, namely donut-shaped (350 V) and...
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my.uthm.eprints.95152023-08-02T03:43:42Z http://eprints.uthm.edu.my/9515/ In vitro surface efficacy of CaP-based anodised titanium for bone implants Alipal, J. Saidin, S. Kai Lo, A.Z. Koshy, P. Abdullah, H.Z. Idris, M.I. Lee, T.C. T Technology (General) This study examined the effect of changes to the surface of CaP-based anodised titanium on osteogenesis and antimicrobial activity in vitro. The present work is proposed to investigate the mechanical stability of two different CaP/TiO2 micromorphology-based coatings, namely donut-shaped (350 V) and needle-shaped (450 V), that are fabricated by single-step micro-arc anodic oxidation at different conditions alongside with their cytocompatibility and antibacterial properties. The microhardness of the sample anodised at 450 V is ~100 MPa lower than that seen in the sample fabricated at 350 V, but both resultant coatings were strongly adhere to the substrate. However, the coating at 450 V was observed to suffer from delamination, indicating the needle-like structure is mechanically unstable. After 7 days of SBF immersion, the 450 V sample was highly bioactive relative to the 350 V sample. However, MTT assays showed that the 450 V coatings showed lower growth rate of hFOB 1.19 at day 14, which indicates that although the structure does have a superior ability to form apatite, it has long-term negative implications possibly from the toxicity of the structure to bone growth. The Alizarin-red staining shows that 450 V sample shows a negative trend of osteogenic mineralisation, thus qualitatively validating the toxicity. For antimicrobial effects, the donut-shaped microstructures were shown to have adequate antibacterial properties compared to the needle-like structures. Overall, this study suggest that the donut-shaped TiO2 morphology is the best cementless interface for bone cell anchorage. Elsevier 2023 Article PeerReviewed text en http://eprints.uthm.edu.my/9515/1/J16043_3ebf01813a855fc8bfbea2ea2e595eb8.pdf Alipal, J. and Saidin, S. and Kai Lo, A.Z. and Koshy, P. and Abdullah, H.Z. and Idris, M.I. and Lee, T.C. (2023) In vitro surface efficacy of CaP-based anodised titanium for bone implants. Surfaces and Interfaces, 39. pp. 1-21. https://doi.org/10.1016/j.surfin.2023.102872 |
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T Technology (General) Alipal, J. Saidin, S. Kai Lo, A.Z. Koshy, P. Abdullah, H.Z. Idris, M.I. Lee, T.C. In vitro surface efficacy of CaP-based anodised titanium for bone implants |
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This study examined the effect of changes to the surface of CaP-based anodised titanium on osteogenesis and
antimicrobial activity in vitro. The present work is proposed to investigate the mechanical stability of two different CaP/TiO2 micromorphology-based coatings, namely donut-shaped (350 V) and needle-shaped (450 V), that are fabricated by single-step micro-arc anodic oxidation at different conditions alongside with their cytocompatibility and antibacterial properties. The microhardness of the sample anodised at 450 V is ~100 MPa lower than that seen in the sample fabricated at 350 V, but both resultant coatings were strongly adhere to the substrate. However, the coating at 450 V was observed to suffer from delamination, indicating the needle-like
structure is mechanically unstable. After 7 days of SBF immersion, the 450 V sample was highly bioactive
relative to the 350 V sample. However, MTT assays showed that the 450 V coatings showed lower growth rate of
hFOB 1.19 at day 14, which indicates that although the structure does have a superior ability to form apatite, it
has long-term negative implications possibly from the toxicity of the structure to bone growth. The Alizarin-red staining shows that 450 V sample shows a negative trend of osteogenic mineralisation, thus qualitatively validating the toxicity. For antimicrobial effects, the donut-shaped microstructures were shown to have adequate antibacterial properties compared to the needle-like structures. Overall, this study suggest that the donut-shaped TiO2 morphology is the best cementless interface for bone cell anchorage. |
format |
Article |
author |
Alipal, J. Saidin, S. Kai Lo, A.Z. Koshy, P. Abdullah, H.Z. Idris, M.I. Lee, T.C. |
author_facet |
Alipal, J. Saidin, S. Kai Lo, A.Z. Koshy, P. Abdullah, H.Z. Idris, M.I. Lee, T.C. |
author_sort |
Alipal, J. |
title |
In vitro surface efficacy of CaP-based anodised titanium for bone implants |
title_short |
In vitro surface efficacy of CaP-based anodised titanium for bone implants |
title_full |
In vitro surface efficacy of CaP-based anodised titanium for bone implants |
title_fullStr |
In vitro surface efficacy of CaP-based anodised titanium for bone implants |
title_full_unstemmed |
In vitro surface efficacy of CaP-based anodised titanium for bone implants |
title_sort |
in vitro surface efficacy of cap-based anodised titanium for bone implants |
publisher |
Elsevier |
publishDate |
2023 |
url |
http://eprints.uthm.edu.my/9515/1/J16043_3ebf01813a855fc8bfbea2ea2e595eb8.pdf http://eprints.uthm.edu.my/9515/ https://doi.org/10.1016/j.surfin.2023.102872 |
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13.18916 |