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Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates

Cholera is a major health issue, affecting millions of lives annually. In light of the recurrent outbreaks of cholera, there is a pressing need for the development of vaccines that allow rapid mass vaccination. In this study, genetically modified vaccine candidates, VCUSM21P and VCUSM22P, were desig...

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Main Author: Murugaiah, Chandrika
Format: Thesis
Language:English
Published: 2011
Subjects:
R5-920 Medicine (General)
Online Access:http://eprints.usm.my/43233/1/CHANDRIKA%20MURUGAIAH.pdf
http://eprints.usm.my/43233/
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spelling my.usm.eprints.43233 http://eprints.usm.my/43233/ Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates Murugaiah, Chandrika R5-920 Medicine (General) Cholera is a major health issue, affecting millions of lives annually. In light of the recurrent outbreaks of cholera, there is a pressing need for the development of vaccines that allow rapid mass vaccination. In this study, genetically modified vaccine candidates, VCUSM21P and VCUSM22P, were designed. VCUSM21P is a prototrophic vaccine which encodes non-toxic cholera toxin A (ctxA) subunit immunogen and has accessory cholera enterotoxin (ace), zonula occludens toxin (zot) and repeats-in-toxin C/A (rtxC/A) mutations. On the other hand, VCUSM22P is ace, zot, ctxA, rtxC/A and haemagglutinin/protease (hap) mutant. Both mutants were found not to disassemble the actin of HEp2 cells. 2011-09 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/43233/1/CHANDRIKA%20MURUGAIAH.pdf Murugaiah, Chandrika (2011) Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates. PhD thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R5-920 Medicine (General)
spellingShingle R5-920 Medicine (General)
Murugaiah, Chandrika
Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates
description Cholera is a major health issue, affecting millions of lives annually. In light of the recurrent outbreaks of cholera, there is a pressing need for the development of vaccines that allow rapid mass vaccination. In this study, genetically modified vaccine candidates, VCUSM21P and VCUSM22P, were designed. VCUSM21P is a prototrophic vaccine which encodes non-toxic cholera toxin A (ctxA) subunit immunogen and has accessory cholera enterotoxin (ace), zonula occludens toxin (zot) and repeats-in-toxin C/A (rtxC/A) mutations. On the other hand, VCUSM22P is ace, zot, ctxA, rtxC/A and haemagglutinin/protease (hap) mutant. Both mutants were found not to disassemble the actin of HEp2 cells.
format Thesis
author Murugaiah, Chandrika
author_facet Murugaiah, Chandrika
author_sort Murugaiah, Chandrika
title Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates
title_short Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates
title_full Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates
title_fullStr Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates
title_full_unstemmed Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates
title_sort construction and evaluation of multigene mutants of vibrio cholerae o139 as vaccine candidates
publishDate 2011
url http://eprints.usm.my/43233/1/CHANDRIKA%20MURUGAIAH.pdf
http://eprints.usm.my/43233/
_version_ 1643710695322157056
score 13.160551

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