Effects of Droplet-Vitrification Cryopreservation Based on Physiological and Antioxidant Enzyme Activities of Brassidium Shooting Star Orchid

• Main Authors: Rahmah, Safrina, Ahmad Mubbarakh, Safiah, Khor, Soo Ping, Subramaniam, Sreeramanan
• Format: Article
• Language: English
• Published: Hindawi Publishing Corporation 2015
• Subjects: QH1 Natural history (General - Including nature conservation, geographical distribution)
• Online Access: http://eprints.usm.my/38896/1/Effects_of_Droplet-Vitrification_Cryopreservation.pdf; http://eprints.usm.my/38896/; http://dx.doi.org/10.1155/2015/961793

Protocorm-like bodies (PLBs) of Brassidium Shooting Star orchid were successfully cryopreserved using droplet-vitrification method. Vitrification based cryopreservation protocol is comprised of preculture, osmoprotection, cryoprotection, cooling, rewarming, and growth recovery and each and every s...