Effects of Droplet-Vitrification Cryopreservation Based on Physiological and Antioxidant Enzyme Activities of Brassidium Shooting Star Orchid
Protocorm-like bodies (PLBs) of Brassidium Shooting Star orchid were successfully cryopreserved using droplet-vitrification method. Vitrification based cryopreservation protocol is comprised of preculture, osmoprotection, cryoprotection, cooling, rewarming, and growth recovery and each and every s...
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Online Access: | http://eprints.usm.my/38896/1/Effects_of_Droplet-Vitrification_Cryopreservation.pdf http://eprints.usm.my/38896/ http://dx.doi.org/10.1155/2015/961793 |
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my.usm.eprints.38896 http://eprints.usm.my/38896/ Effects of Droplet-Vitrification Cryopreservation Based on Physiological and Antioxidant Enzyme Activities of Brassidium Shooting Star Orchid Rahmah, Safrina Ahmad Mubbarakh, Safiah Khor, Soo Ping Subramaniam, Sreeramanan QH1 Natural history (General - Including nature conservation, geographical distribution) Protocorm-like bodies (PLBs) of Brassidium Shooting Star orchid were successfully cryopreserved using droplet-vitrification method. Vitrification based cryopreservation protocol is comprised of preculture, osmoprotection, cryoprotection, cooling, rewarming, and growth recovery and each and every step contributes to the achievement of successful cryopreservation. In order to reveal the lethal and nonlethal damage produced by cryopreservation, histological observation, scanning electron microscopy (SEM), and biochemical analysis were carried out in both cryopreserved and noncryopreserved PLBs of Brassidium Shooting Star orchid comparing with the control PLBs stock culture. Histological and scanning electron microscopy analyses displayed structural changes in cryopreserved PLBs due to the impact of cryoinjury during exposure to liquid nitrogen. Total soluble protein significantly increased throughout the dehydration process and the highest value was achieved when PLBs were stored in liquid nitrogen. Ascorbate peroxidase (APX) and catalase (CAT) showed the highest enzyme activities in both dehydration and cryostorage treatments indicating that stress level of PLBs was high during these stages. Hindawi Publishing Corporation 2015 Article PeerReviewed application/pdf en http://eprints.usm.my/38896/1/Effects_of_Droplet-Vitrification_Cryopreservation.pdf Rahmah, Safrina and Ahmad Mubbarakh, Safiah and Khor, Soo Ping and Subramaniam, Sreeramanan (2015) Effects of Droplet-Vitrification Cryopreservation Based on Physiological and Antioxidant Enzyme Activities of Brassidium Shooting Star Orchid. Scientific World Journal, 2015 (961793). pp. 1-10. ISSN 2356-6140 http://dx.doi.org/10.1155/2015/961793 |
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QH1 Natural history (General - Including nature conservation, geographical distribution) Rahmah, Safrina Ahmad Mubbarakh, Safiah Khor, Soo Ping Subramaniam, Sreeramanan Effects of Droplet-Vitrification Cryopreservation Based on Physiological and Antioxidant Enzyme Activities of Brassidium Shooting Star Orchid |
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Protocorm-like bodies (PLBs) of Brassidium Shooting Star orchid were successfully cryopreserved using droplet-vitrification
method. Vitrification based cryopreservation protocol is comprised of preculture, osmoprotection, cryoprotection, cooling,
rewarming, and growth recovery and each and every step contributes to the achievement of successful cryopreservation. In order
to reveal the lethal and nonlethal damage produced by cryopreservation, histological observation, scanning electron microscopy
(SEM), and biochemical analysis were carried out in both cryopreserved and noncryopreserved PLBs of Brassidium Shooting
Star orchid comparing with the control PLBs stock culture. Histological and scanning electron microscopy analyses displayed
structural changes in cryopreserved PLBs due to the impact of cryoinjury during exposure to liquid nitrogen. Total soluble
protein significantly increased throughout the dehydration process and the highest value was achieved when PLBs were stored
in liquid nitrogen. Ascorbate peroxidase (APX) and catalase (CAT) showed the highest enzyme activities in both dehydration and
cryostorage treatments indicating that stress level of PLBs was high during these stages. |
format |
Article |
author |
Rahmah, Safrina Ahmad Mubbarakh, Safiah Khor, Soo Ping Subramaniam, Sreeramanan |
author_facet |
Rahmah, Safrina Ahmad Mubbarakh, Safiah Khor, Soo Ping Subramaniam, Sreeramanan |
author_sort |
Rahmah, Safrina |
title |
Effects of Droplet-Vitrification Cryopreservation Based on
Physiological and Antioxidant Enzyme Activities of Brassidium
Shooting Star Orchid |
title_short |
Effects of Droplet-Vitrification Cryopreservation Based on
Physiological and Antioxidant Enzyme Activities of Brassidium
Shooting Star Orchid |
title_full |
Effects of Droplet-Vitrification Cryopreservation Based on
Physiological and Antioxidant Enzyme Activities of Brassidium
Shooting Star Orchid |
title_fullStr |
Effects of Droplet-Vitrification Cryopreservation Based on
Physiological and Antioxidant Enzyme Activities of Brassidium
Shooting Star Orchid |
title_full_unstemmed |
Effects of Droplet-Vitrification Cryopreservation Based on
Physiological and Antioxidant Enzyme Activities of Brassidium
Shooting Star Orchid |
title_sort |
effects of droplet-vitrification cryopreservation based on
physiological and antioxidant enzyme activities of brassidium
shooting star orchid |
publisher |
Hindawi Publishing Corporation |
publishDate |
2015 |
url |
http://eprints.usm.my/38896/1/Effects_of_Droplet-Vitrification_Cryopreservation.pdf http://eprints.usm.my/38896/ http://dx.doi.org/10.1155/2015/961793 |
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1643709492124188672 |
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