A Potential Epigenetic Marker Mediating Serum Folate and Vitamin B12 Levels Contributes to the Risk of Ischemic Stroke
Stroke is a multifactorial disease that may be associated with aberrant DNA methylation profiles. We investigated epigenetic dysregulation for themethylenetetrahydrofolate reductase (MTHFR) gene among ischemic stroke patients. Cases and controls were recruited after obtaining signed written inform...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2015
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| Subjects: | |
| Online Access: | http://eprints.usm.my/38095/1/A_Potential_Epigenetic_Marker_Mediating_Serum_Folate_and_Vitamin_B12_Levels_Contributes_to_the.pdf http://eprints.usm.my/38095/ http://dx.doi.org/10.1155/2015/167976 |
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| Summary: | Stroke is a multifactorial disease that may be associated with aberrant DNA methylation profiles. We investigated epigenetic
dysregulation for themethylenetetrahydrofolate reductase (MTHFR) gene among ischemic stroke patients. Cases and controls were
recruited after obtaining signed written informed consents following a screening process against the inclusion/exclusion criteria.
Serum vitamin profiles (folate, vitamin B12, and homocysteine) were determined using immunoassays. Methylation profiles for
CpGs A and B in the MTHFR gene were determined using a bisulfite-pyrosequencingmethod.Methylation of MTHFR significantly
increased the susceptibility risk for ischemic stroke. In particular, CpG A outperformed CpG B in mediating serum folate and
vitamin B12 levels to increase ischemic stroke susceptibility risks by 4.73-fold. However, both CpGs A and B were not associated
with serum homocysteine levels or ischemic stroke severity. CpG A is a potential epigenetic marker in mediating serum folate and
vitamin B12 to contribute to ischemic stroke. |
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