Physicochemical Properties And Prebiotic Potential Of Native, Resistant And Hcl-Resistant Starches From Sago (Metroxylon Sagu)
Kanji rintang jenis III (RS3) telah dihasilkan daripada sagu (Metroxylon sagu) dan dinilai sifat fizikokimia and potensinya sebagai prebiotik. Sampel mengandungi 35.7% kanji rintang (dikenal sebagai sagu RS) telah dihasilkan apabila kanji sagu asli diautoklaf dalam air suling pada suhu 121 °C sel...
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Format: | Thesis |
Language: | English |
Published: |
2016
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Online Access: | http://eprints.usm.my/31864/1/TAN_ZI_NI_24%28NN%29.pdf http://eprints.usm.my/31864/ |
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Summary: | Kanji rintang jenis III (RS3) telah dihasilkan daripada sagu (Metroxylon sagu)
dan dinilai sifat fizikokimia and potensinya sebagai prebiotik. Sampel mengandungi
35.7% kanji rintang (dikenal sebagai sagu RS) telah dihasilkan apabila kanji sagu asli
diautoklaf dalam air suling pada suhu 121 °C selama 1 jam, dinyahcabang dengan 20
U pullulanase per g kanji pada 60 °C selama 24 jam dan seterusnya diautoklaf sekali
lagi pada 121 °C selama 1 jam sebelum disimpan pada 4 °C selama 24 jam.
Seterusnya, kandungan kanji rintang meningkat sehingga 63.8% (sampel dikenal
sebagai HCl-sagu RS) selepas sagu RS dihidrolisiskan dengan 0.5 M HCl pada suhu
60 °C. Granul sagu RS dan HCl-sagu RS menunjukkan corak pembelauan sinar X
jenis B, suhu puncak yang tinggi (143.7 °C and 146.5 ºC, masing-masing) dan
struktur permukaan yang tidak sekata dan kasar. Granul kanji sagu asli menunjukkan
corak pembelauan sina X jenis C, suhu puncak 74.6 °C dan permukaan yang sekata.
Keterlarutan dan kuasa pembengkakan sampel HCl-sagu RS ialah 14.9% dan 1.94
g/g, masing-masing, iaitu lebih rendah berbanding sagu RS (27.4% and 2.82 g/g,
masing-masing).
Resistant starch type III (RS3) was produced from sago (Metroxylon sagu)
and evaluated for its physicochemical properties and potential as a prebiotic. A
sample with 35.7% RS3 content (designated as sago RS) was produced when the
native sago starch was suspended in distilled water, gelatinized by autoclaving at
121 °C for 1 h, followed by debranching with 20 U pullulanase per g starch at 60 °C
for 24 h, autoclaved again at 121 °C for 1 h before storage at 4 °C for 24 h. RS3
content was further increased with the treatment of sago RS with 0.5 M HCl at 60 °C
(sample designated HCl-sago RS) to 63.8%. Granules of sago RS and HCl-sago RS
had B-type X-ray diffraction pattern, high peak temperatures (143.7 °C and 146.5 ºC,
respectively) and showed irregular and rough surface structure. While granules of
native sago starch had C-type diffraction pattern, peak temperature of 74.6 °C and
smooth granular surface. The solubility and the swelling power of HCl-sago RS
samples were 14.9% and 1.94 g/g, respectively, which were lower than that of sago
RS (27.4% and 2.82 g/g, respectively). |
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