Effects Of Cymbopogon Sp. Essential Oils And Streptomycin Sulfate On Antimicrobial Proteins Production By Bacillus Subtilis Atcc 21332
Proteins level produced by bacteria may be increased in stressful surroundings, such as in the presence of antimicrobial agent. It appears that many antimicrobial agents, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from...
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| Format: | Newspaper Article |
| Language: | English |
| Published: |
Universiti Sains Islam Malaysia
2015
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| Subjects: | |
| Online Access: | http://ddms.usim.edu.my/handle/123456789/8629 |
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| Summary: | Proteins level produced by bacteria may be increased in stressful surroundings, such
as in the presence of antimicrobial agent. It appears that many antimicrobial agents,
when used at low concentrations, have in common the ability to activate or repress
gene transcription, which is distinct from their inhibitory effect. However, there have
been comparatively few studies on the potential of natural compounds as a specific
chemical signal that can trigger a variety of biological functions. Therefore, the aim of this study is to evaluate the effects of Cymbopogon sp. (which are C. nardus and C.
flexuosus) essential oils and Streptomycin Sulfate in regulating proteins or peptides
production by Bacillus subtilis ATCC 21332. Results of Microdilution assay showed
that the Minimum Inhibition Concentrations (MICs) of C. nardus and C. flexuosus as
well as Streptomycin Sulfate on B. subtilis ATCC 21332 were 1.56% (v/v), 0.2% (v/v)
and 2.5 mg/ml respectively. C. flexuosus and C. nardus essential oils at concentration
of 0.01 MIC, each was added during early log phase of bacterial growth at 37ºC,
resulting the production of new intracellular proteins with similar approximate size of 180 kDa in which recognized as DNA-directed RNA polymerase β subunit enzyme and respiratory nitrate reductase α subunit enzyme respectively. Besides, when B. subtilis ATCC 21332 were induced by C. flexuosus essential oil and Streptomycin Sulfate during log phase of growing cells at 30ºC could also secrete the extracellular proteins with approximate size of 30 kDa, identified as Bacillopeptidase F. In addition, B. subtilis ATCC 21332 in the presence of either C. nardus or C. flexuosus
essential oils could secrete the bioactive extracellular proteins with potent
antimicrobial activity against certain Gram-positive and Gram-negative bacteria.
Hence, B. subtilis ATCC 21332 in stressful condition with the presence of either
Cymbopogon sp. essential oils or Streptomycin Sulfate at 0.01 MIC level were able to induce the production or secretion of bioactive proteins. |
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