Evaluation of total phenolic content and antioxidant activities from different type of extraction technique of Helianthus tuberosus

• Main Authors: Alias, Nur Diyana, Puteh, Fadzilah, Mohd Zulkifli, Nurin Izzati, Noor Hasnan, Noor Zafira
• Format: Article
• Language: English
• Published: HH Publisher 2021
• Online Access: http://psasir.upm.edu.my/id/eprint/97175/1/ABSTRACT.pdf; http://psasir.upm.edu.my/id/eprint/97175/; https://journals.hh-publisher.com/index.php/AAFRJ/article/view/259

This study investigated the difference of antioxidants activity from different extraction techniques of Jerusalem artichoke (Helianthus tuberosus). Three different extraction techniques involved were aqueous extraction, high-pressure steam extraction and 70% ethanol extraction. Total phenolic content (TPC) was determined using the Folin-Ciocalteu method. The antioxidant activities were analysed using ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). The high-pressure steam extract showed the highest total phenolic content and both FRAP and DPPH activities at 127.87 ± 0.08 mg GAE/g, 53.38 ± 2.76 mg AAE/g and 79.92 ± 0.26 mg AAE/g. Meanwhile, the aqueous extract showed 2-fold higher of total phenolic content compared to ethanol extract at 65.73 ± 9.44 and 30.61 ± 0.00 mg GAE/g. Conversely, antioxidant activities obtained from ethanol extracts (41.49 ± 2.54 and 41.10 ± 0.10 mg AAE/g.) were higher compared to aqueous extract (23.29 ± 0.47 and 5.24 ± 0.38 mg AAE/g.). This study suggests that different extraction methods influenced the findings of total phenolic content and antioxidant activities of the Jerusalem artichoke. Hence, Jerusalem artichoke can be considered as a potential source of natural antioxidants.